枸杞的抗炎活性成分及作用机理  

Anti-inflammatory active ingredients and mechanisms of action of Lycii fructus

作　　者：史小飞 SHI Xiaofei(Neihuang County Market Supervision Administration,Anyang,Henan 456399,China)

机构地区：[1]内黄县市场监督管理局,河南安阳456399

出　　处：《食品与机械》2025年第3期151-160,共10页Food and Machinery

摘　　要：[目的]探究枸杞的抗炎活性成分及作用机理。[方法]运用网络药理学和分子对接技术筛选枸杞抗炎作用的主要成分及作用靶点,结合细胞试验,以RAW264.7炎症细胞模型评价枸杞提取物的抗炎活性,并利用皮尔逊相关系数法分析主要成分与炎症因子之间的关联性。[结果]通过TCMSP、Uniprot、OMIM等在线数据库筛选出34种枸杞主要成分及123个共有靶点基因,由蛋白互作网络图收集到IL-6、TP53、JUN、TNF、AKT1、IL1B等核心靶点,由“药物—成分—靶点”网络图可获得槲皮素、β-谷甾醇、黄豆黄素、豆甾醇、阿托品等关键成分;KEGG富集得到了癌症信号通路、脂质和动脉粥样硬化等信号通路。分子对接结果表明,β-谷甾醇、豆甾醇与关键靶点的结合能相对较低,亲和力较强。细胞试验进一步验证了枸杞提取物中槲皮素、β-谷甾醇、豆甾醇浓度与炎症因子TNF-α、IL-6、IL-1β生成量之间存在负相关性。[结论]枸杞中潜在的抗炎活性成分为槲皮素、β-谷甾醇、豆甾醇,可能是通过调节AKT1、IL1B等核心靶点来发挥抗炎作用。[Objective]To explore the anti-inflammatory active ingredients and mechanisms of action of Lycii fructus.[Methods]Network pharmacology and molecular docking techniques were used to screen the main components and targets of Lycii fructus with antiinflammatory effects.Combined with cell experiments,the anti-inflammatory activity of Lycii fructus extract was evaluated using the RAW264.7 inflammatory cell model.The Pearson correlation coefficient method was used to analyze the correlation between the main components and inflammatory factors.[Results]A total of 34 main components and 123 common target genes of Lycii fructus were screened through online databases such as TCMSP,Uniprot,and OMIM.Core targets,including IL-6,TP53,JUN,TNF,AKT1,and IL1B,were identified through the protein-protein interaction network.Key components such as quercetin,β-sitosterol,daidzein,stigmasterol,and atropine were obtained from"drug-component-target"network.KEGG pathway enrichment analysis revealed cancer signaling pathway,lipid signaling pathway,and atherosclerosis signaling pathway.Molecular docking results showed thatβ-sitosterol and stigmasterol exhibited relatively low binding energies and strong affinities with key targets.Cell experiments further confirmed a negative correlation between the concentrations of quercetin,β-sitosterol,and stigmasterol in Lycii fructus extract and the production of inflammatory factors TNF-α,IL-6,and IL-1β.[Conclusion]The potential anti-inflammatory active ingredients in Lycii fructus are quercetin,β-sitosterol,and stigmasterol,which may exert anti-inflammatory effects by regulating core targets such as AKT1 and IL1B.

关 键 词：枸杞 活性成分 靶点 活性 抗炎 

分 类 号：R285[医药卫生—中药学]