红花多糖纯化工艺及体外抗氧化活性研究  

Purification process and antioxidant activity in vitro of polysaccharides from safflower

作　　者：张馨心 王福玲[1,3] ZHANG Xinxin;WANG Fuling(School of Pharmacy,Harbin University of Commerce,Harbin,Heilongjiang 150076,China;Honors'School,Harbin University of Commerce,Harbin,Heilongjiang 150076,China;Zhejiang Shangyao Jiuxu Pharmaceutical Co.,Ltd.,Jinhua,Zhejiang 321016,China)

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076 [2]哈尔滨商业大学英才学院,黑龙江哈尔滨150076 [3]浙江上药九旭药业有限公司,浙江金华321016

出　　处：《食品与机械》2025年第3期171-176,共6页Food and Machinery

基　　金：国家级大学生创新创业训练计划项目(编号:202210240022)。

摘　　要：[目的]采用水提醇沉法提取红花多糖(safflower polysaccharides,SPS),并分析其结构和体外抗氧化活性。[方法]红花粗多糖经脱蛋白、DEAE-52纤维素和Sephadex G-200凝胶分离纯化后,得到SPS-A、SPS-B和SPS-C 3个组分,采用紫外—可见光谱扫描和红外光谱对其进行分析,并考察SPS-A的体外抗氧化能力。[结果]SPS-A、SPS-B和SPS-C含量分别为89.75%,90.37%,90.22%,样品中不含蛋白质或核酸类物质,推测SPS-A、SPS-B、SPS-C为β-型吡喃环结构。SPS-A清除DPPH自由基的IC50值为0.35 mg/m L,Fe^(3+)总抗氧化能力(FRAP)为411.58μmol/L FeSO_(4)。[结论]木瓜蛋白酶-Sevag法对红花粗多糖的脱蛋白效果最佳;红花多糖可能含β-型吡喃环结构;红花多糖可作为一种新型抗氧化药物及食物开发。[Objective]This study aimed to extract Safflower polysaccharides(SPS)via water extraction and alcohol precipitation method,and to analyze their structural characteristics and antioxidant activity in vitro.[Methods]SPS was separated into three fractions-SPS-A,SPS-B and SPS-C using DEAE-52 ion exchange and Sephadex G-200 gel chromatography.Structural properties were analyzed through UVvis scanning and infrared spectroscopy.The antioxidant activity of SPS-A was assessed through DPPH radical scavenging and ferric reducing antioxidant power(FRAP)assays.[Results]The polysaccharide contents of SPS-A,SPS-B and SPS-C was 89.75%,90.37%,90.22%,respectively.The samples did not contain protein or nucleic acid substances.It is speculated that SPS-A,SPS-B and SPS-C wereβ-type pyran ring structures.The IC50 value of SPS-A for scavenging DPPH free radicals was 0.35 mg/mL,and the total antioxidant capacity of Fe^(3+)(FRAP)was 411.58μmol/L FeSO_(4).[Conclusion]The papain-Sevag method was the most effective for deproteinization of crude polysaccharides.Safflower polysaccharide,characterized byβ-type pyranose ring structures,demonstrated significant antioxidant potential,which can be enhanced by increasing polysaccharide concentration.Optimizing separation and purification processes could improve sample yield and support large-scale production.SPS holds promise as a novel antioxidant for pharmaceutical and functional food applications.

关 键 词：红花多糖 分离纯化 柱层析 红外光谱 抗氧化 

分 类 号：R284.2[医药卫生—中药学]