核酸合成仪校准方法  

Research on calibration method of nucleic acid synthesizer

作　　者：梁文[1] 杨镇州 林正皓 杨雪 李杰[1] 李亮[2] 闻艳丽[1] 霍银波 Liang Wen;Yang Zhenzhou;Lin Zhenghao;Yang Xue;Li Jie;Li Liang;Wen Yanli;HuoYinbo(Shanghai Institute of Measurement and Testing Technology;Chinese Academy of Agricultural Sciences)

机构地区：[1]上海市计量测试技术研究院 [2]中国农业科学院

出　　处：《上海计量测试》2025年第1期13-16,共4页Shanghai Measurement and Testing

基　　金：国家自然科学基金(22074093)。

摘　　要：根据核酸合成仪的工作原理和应用情况,介绍了仪器的压力示值误差、压力泄漏速率、进样体积误差、进样体积重复性、目标序列核酸分子量相对误差和目标序列核酸纯度的校准方法和校准结果,探讨了这六个技术指标对仪器性能评估的重要性,分析了评估过程的技术难点,为核酸合成仪质量状况评估及相关校准规范的制定提供了参考依据。Based on the working principle and application of the nucleic acid synthesizer,this paper focuses on the detection and comparison of six technical indicators,including pressure gauge error,pressure leakage rate,sample volume error,sample volume repeatability,relative error of target nucleic acid molecular weight,and purity of target nucleic acid,to discuss the importance of these six technical indicators in evaluating the performance of the instrument,analyze the technical difficulties in the evaluation process,and provide reference basis for evaluating the quality status of nucleic acid synthesizers and formulating relevant calibration specifications.

关 键 词：核酸合成仪 仪器校准 技术指标 

分 类 号：TH83[机械工程—仪器科学与技术]