痰瘀互结证急性冠脉综合征大鼠肠道菌群的宏基因组测序探析  

Metagenomic sequencing analysis of intestinal flora in rats with acute coronary syndrome and intertwined phlegm and stasis syndrome

作　　者：陈彦熹 张妮 CHEN Yan-xi;ZHANG Ni(School of First Clinical,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学第一临床学院,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《时珍国医国药》2025年第5期976-984,共9页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金青年基金(82104841);辽宁省教育厅青年科技人才“育苗”项目(L202039)。

摘　　要：目的基于宏基因组测序探析痰瘀互结证急性冠脉综合征(acute coronary syndrome,ACS)大鼠的肠道菌群特征,为临床防治提供科学实验依据。方法采用高脂饮食联合腹腔注射异丙肾上腺素建立痰瘀互结证ACS模型,宏基因组测序分析大鼠肠道菌群物种组成,并进行Alpha多样性指数分析、LEfSe多级物种差异判别分析、KEGG功能注释及Network分析。结果与正常组相比,痰瘀互结证ACS组放线菌门、变形菌门、螺旋体门、梭菌属等丰度升高,乳杆菌属等丰度降低,差异菌群为变形菌门、放线菌门、密螺旋体属、柯林斯菌属等,差异代谢通路为苯丙氨酸、酪氨酸和色氨酸的生物合成、缬氨酸、亮氨酸和异亮氨酸的生物合成等。正常组大鼠的差异菌群为乳杆菌属等,差异代谢通路为半乳糖代谢等。放线菌门、变形菌门、螺旋体门与苯丙氨、酸酪氨酸和色氨酸的生物合成、精氨酸的生物合成、缬氨酸、亮氨酸和异亮氨酸的生物合成等成显著正相关(P<0.05)。结论痰瘀互结证ACS组大鼠特征性差异菌群为变形菌门、密螺旋体属、柯林斯菌属等,变形菌门等与苯丙氨酸、酪氨酸和色氨酸的生物合成等代谢通路显著相关,差异菌群通过协同调节差异通路可能是痰瘀互结证急性冠脉综合征发生发展的关键病机。Objective To characterize the intestinal flora of rats with acute coronary syndrome with phlegm and blood stasis based on metagenomic next-generation sequencing,and to provide a scientific and experimental basis for clinical prevention and treatment.Methods An ACS model with intertwined phlegm and stasis syndrome was established by feeding high-fat diet combined with intraperitoneal injection of isoproterenol.Intestinal flora composition was analyzed by metagenomic sequencing,followed by Alpha diversity analysis,LEfSe multi-level species discriminant analysis,KEGG functional annotation,and Network analysis.Results Compared to the normal group,the ACS group showed increased abundances of Actinobacteria,Proteobacteria,Spirochaete and Clostridium,but decreased Lactobacillus.Differential flora included Proteobacteria,Actinobacteria,Treponema,Collinsella,while differential metabolic pathways involved biosynthesis of phenylalanine,tyrosine,tryptophan,valine,leucine,and isoleucine.In the normal group,differential flora was Lactobacillus,and the differential metabolic pathway was galactose metabolism.Actinobacteria,Proteobacteria,and Spirochaetae showed significant positive correlations with biosynthesis pathways of phenylalanine,tyrosine,tryptophan,arginine,valine,leucine,and isoleucine(P<0.05).Conclusion The characteristic differential microbiota in ACS rats with intertwined phlegm and stasis syndrome included Proteobacteria,Treponema and Collinsella.Proteobacteria was significantly associated with biosynthesis pathways of phenylalanine,tyrosine and tryptophan.The differential flora may be the key pathogenesis of the occurrence and development of ACS and phlegm stasis interjunction syndrome through synergistic regulation of the differential pathways.

关 键 词：肠道菌群 痰瘀互结证 急性冠脉综合征 宏基因组测序 

分 类 号：R259[医药卫生—中西医结合] R256.2[医药卫生—中医内科学]