火麻仁油通过调控肠道菌群改善非酒精性脂肪性肝炎的作用机制研究  

Study on the Mechanism of Hemp Seed Oil in Ameliorating Non-alcoholic Steatohepatitis by Regulating Gut Microbiota

作　　者：杨静 张玉杰 邹忠杰 龚梦鹃 YANG Jing;ZHANG Yujie;ZOU Zhongjie;GONG Mengjuan(School of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006 Guangdong,China)

机构地区：[1]广东药科大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2025年第4期594-601,共8页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省普通高校特色创新类项目(2021KTSCX051)。

摘　　要：目的采用16S rRNA高通量测序技术探讨火麻仁油对非酒精性脂肪性肝炎(NASH)小鼠肠道菌群的影响。方法将18只C57BL/6J雄性小鼠随机分为正常组、模型组和火麻仁油组(1 g·kg^(-1)),每组6只。正常组喂养蛋氨酸-胆碱充足(MCS)饲料,其余两组喂养蛋氨酸-胆碱缺乏(MCD)饲料4周复制NASH小鼠模型。模型复制期间火麻仁油组灌胃1 g·kg^(-1)火麻仁油,其余两组给予等体积0.5%吐温-80。实验过程中记录各组小鼠体质量,4周后收集血清、肝脏和粪便样本。采用苏木精-伊红(HE)、Masson和油红O染色法观察肝脏组织病理损伤;采用酶联免疫吸附测定法(ELISA)测定血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的含量;采用16S rRNA高通量测序技术检测小鼠肠道菌群的变化。结果与正常组比较,模型组小鼠的体质量明显降低(P<0.01),血清ALT和AST的含量明显升高(P<0.001),肝脏内出现大量脂滴堆积、脂肪变性,伴有大量炎性细胞浸润,且肝脏发生纤维化。与模型组比较,火麻仁油能够明显改善NASH小鼠的体质量减轻(P<0.05),明显降低血清中ALT和AST的水平(P<0.01),并能显著改善肝脏病理损伤。16S rRNA测序结果显示,火麻仁油可以逆转NASH小鼠肠道菌群Alpha与Beta多样性的下降。此外,鉴定了19种与NASH相关的菌属,火麻仁油可以对其中7种产生有效的调节。结论火麻仁油可以改善NASH小鼠肝组织的脂滴堆积、脂质变性、炎症反应和纤维化,其作用机制可能是通过调节肠道菌群多样性,促进有益菌和抑制有害菌的生长来改善NASH。Objective The effect of hemp seed oil on intestinal flora in non-alcoholic steatohepatitis(NASH)mice was investigated by 16S rRNA high-throughput sequencing technology.Methods Eighteen C57BL/6J male mice were randomly divided into normal group,model group and hemp seed oil group(1 g·kg^(-1)),with 6 mice in each group.The normal group was fed with methionine-choline sufficiency(MCS)diet,and the other two groups were fed with methionine-choline deficient(MCD)diet for 4 weeks to replicate NASH mouse model.During the model replication,the hemp seed oil group was given 1 g·kg^(-1)hemp seed oil,and the other two groups were given equal volume of 0.5%Tween-80.The body mass of mice in each group was recorded during the experiment.After 4 weeks,serum,liver and fecal samples were collected.The pathological damage of liver tissue was detected by hematoxylin-eosin(HE),Masson and oil red O staining.The contents of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum were determined by enzyme-linked immunosorbent assay(ELISA).The changes of gut microbiota in mice were detected by 16 S rRNA high-throughput sequencing technology.Results Compared with the normal group,the body mass of the mice in the model group was significantly reduced(P<0.01),and the contents of serum ALT and AST were significantly increased(P<0.001).A large number of lipid droplets and steatosis were observed in the liver,accompanied by a large number of inflammatory cell infiltration and liver fibrosis.Compared with the model group,hemp seed oil could significantly improve the body mass loss of NASH mice(P<0.05),significantly reduce the levels of ALT and AST in serum(P<0.01),and significantly improve the pathological damage of liver.The results of 16S rRNA sequencing showed that hemp seed oil could reverse the decrease of Alpha and Beta diversity of gut microbiota in NASH mice.In addition,19 NASH-related genera were identified,and hemp seed oil could effectively regulate 7 of them.Conclusion Hemp seed oil can improve lipid droplet accum

关 键 词：非酒精性脂肪性肝炎 火麻仁油 肠道菌群 16S rRNA高通量测序 小鼠 

分 类 号：R285.5[医药卫生—中药学]