ZmL75 is required for colonization by arbuscular mycorrhizal fungi and for saline-alkali tolerance in maize  

作　　者：Jie Liu Boming Yang Xunji Chen Tengfei Zhang Huairen Zhang Yimo Du Qian Zhao Zhaogui Zhang Darun Cai Juan Liu Huabang Chen Li Zhao 

机构地区：[1]Institute of Genetics and Developmental Biology,Key Laboratory of Seed Innovation,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agriculture/Xinjiang Key Laboratory of Crop Biotechnology,Urumqi,Xinjiang 830091,China

出　　处：《Journal of Genetics and Genomics》2025年第3期334-345,共12页遗传学报(英文版)

基　　金：National Natural Science Foundation of China(No.32171947 and No.31671699)which supported this research.

摘　　要：Saline-alkali soil severely reduces the productivity of crops,including maize(Zea mays).Although several genes associated with saline-alkali tolerance have been identified in maize,the underlying regulatory mechanism remains elusive.Here,we report a direct link between colonization by arbuscular mycorrhizal fungi(AMF)and saline-alkali tolerance in maize.We identify s75,a natural maize mutant that cannot survive under moderate saline-alkali soil conditions or establish AM symbioses.The saline-alkali hypersensitive phenotype of s75 is caused by a 1340-bp deletion in Zm00001d033915,designated as ZmL75.This gene encodes a glycerol-3-phosphate acyltransferase localized in the endoplasmic reticulum,and is responsible for AMF colonization.ZmL75 expression levels in roots correspond with the root length colonization(RLC)rate during early vegetative development.Notably,the s75 mutant line shows a complete loss of AMF colonization,along with alterations in the diversity and structure of its root fungal microbiota.Conversely,overexpression of ZmL75 increases the RLC rate and enhances tolerance to saline-alkali soil conditions.These results suggest that ZmL75 is required for symbiosis with AMF,which directly improves saline-alkali tolerance.Our findings provide insights into maize-AMF interactions and offer a potential strategy for maize improvement.

关 键 词：MAIZE SALINE-ALKALI ZmL75 AMF colonization TOLERANCE 

分 类 号：S513[农业科学—作物学]