ZmGolS1 underlies natural variation of raffinose content and salt tolerance in maize  

作　　者：Xiaoyan Liang Pan Yin Fenrong Li Yibo Cao Caifu Jiang 

机构地区：[1]State Key Laboratory of Plant Environmental Resilience,Frontiers Science Center for Molecular Design Breeding,College of Biological Sciences,China Agricultural University,Beijing 100193,China [2]Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education,College of Forestry,Beijing Forestry University,Beijing 100083,China

出　　处：《Journal of Genetics and Genomics》2025年第3期346-355,共10页遗传学报(英文版)

基　　金：support from the National Key R&D Program of China(2022YFF1001601);the National Science Fund for Distinguished Young Scholars(32325037);the National Natural Science Foundation of China(32201718 and 32401756);the Postdoctoral Innovation Talents Support Program(BX20240420);the China Postdoctoral Science Foundation(2024T171011 and 2023M743817).

摘　　要：Salt stress significantly inhibits crop growth and development,and mitigating this can enhance salt tolerance in various crops.Previous studies have shown that regulating saccharide biosynthesis is a key aspect of plant salt tolerance;however,the underlying molecular mechanisms remain largely unexplored.In this study,we demonstrate that overexpression of a salt-inducible galactinol synthase gene,ZmGolS1,alleviates salt-induced growth inhibition,likely by promoting raffinose synthesis.Additionally,we show that natural variation in ZmGolS1 transcript levels contributes to the diversity of raffinose content and salt tolerance in maize.We further reveal that ZmRR18,a type-B response regulator transcription factor,binds to the AATC element in the promoter of ZmGolS1,with this binding increases the transcript levels of ZmGolS1 under salt conditions.Moreover,a single nucleotide polymorphism(termed SNP-302T)within the ZmGolS1 promoter significantly reduces its binding affinity for ZmRR18,resulting in decreased ZmGolS1 expression and diminished raffinose content,ultimately leading to a salt-hypersensitive phenotype.Collectively,our findings reveal the molecular mechanisms by which the ZmRR18-ZmGolS1 module enhances raffinose biosynthesis,thereby promoting maize growth under salt conditions.This research provides important insights into salt tolerance mechanisms associated with saccharide biosynthesis and identifies valuable genetic loci for breeding salt-tolerant maize varieties.

关 键 词：MAIZE Salt tolerance BIOMASS Raffinose content Transcriptional regulation 

分 类 号：S513[农业科学—作物学]