超高效液相色谱法测定贵州党参中党参炔苷含量  

Determination of Lobetyolin Content in Guizhou Codonopsis Radix by UPLC

作　　者：路利亚 何羽 雷联伟 LU Li-Ya;HE Yu;LEI Lian-Wei(Bijie City market supervision Administration inspection and testing center,Bijie 551700,China)

机构地区：[1]毕节市市场监督管理局检验检测中心,贵州毕节551700

出　　处：《中国药物经济学》2025年第2期89-92,共4页China Journal of Pharmaceutical Economics

基　　金：毕节市创新平台及人才团队科技计划项目(毕科合[2023]16号)。

摘　　要：目的建立超高效液相色谱法(UPLC)测定贵州党参中党参炔苷含量,为贵州党参的质量控制提供方法参考。方法采用Waters CORTECS T_(3)色谱柱;流动相:乙腈-水(含0.1%磷酸)(23∶77);流速:0.4 ml/min;检测波长:268 nm。结果党参炔苷的线性方程Y=3.1963×104X+3.3047×10^(3)(r=0.9993);线性范围:0.01081~1.0808μg,党参炔苷的平均加样回收率为98.87%(n=6)。结论所建立的UPLC法测定贵州党参中党参炔苷含量操作快速简便,结果准确,重复性好,在实际工作中有较强的实用性,可用于贵州党参药材的质量控制。Objective To establish an UPLC method for the determination of lobetyolin in Guizhou Codonopsis Radix and to provide a method reference for the quality control of Guizhou Codonopsis Radix.Methods Waters CORTECS T_(3) chromatographic column was used.Mobile phase:acetonitrile-water(containing 0.1%phosphoric acid)(23∶77),flow rate:0.4 ml/min;the detection wavelength was 268 nm.Results The linear equation of lobetyolin was Y=3.1963×104X+3.3047×10^(3)(r=0.9993).Linear range:0.01081~1.0808μg,the average recovery of lobetyolin was 98.87%(n=6).Conclusion The established UPLC method was used to determine the content of lobetyolin in Guizhou Codonopsis Radix.The operation was fast and simple,the results were accurate,and the repeatability was good.It has strong practicability in practical work and can be used for quality control of Guizhou Codonopsis Radix.

关 键 词：超高效液相色谱法 贵州党参 党参炔苷 质量控制 

分 类 号：R286.0[医药卫生—中药学]