不同强度电针“天枢”对大鼠远端结肠运动的双向调控  

Bidirectional regulation of distal colon motility in rats with electroacupuncture of different intensitiesat"Tianshu"(ST25)

作　　者：李桐 刘筱羽 王晓宇[1] 骆敏 张知云 宿杨帅[1] 景向红[1] LI Tong;LIU Xiaoyu;WANG Xiaoyu;LUO Min;ZHANG Zhiyun;SU Yangshuai;JING Xianghong(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《中国针灸》2025年第4期460-472,共13页Chinese Acupuncture & Moxibustion

基　　金：国家重点研发计划资助项目:2022YFC3500702;国家自然科学基金面上项目:82274245;中国中医科学院科技创新工程重大攻关项目:CI2021A03501;中国中医科学院科技创新工程创新团队项目:CI2021B011。

摘　　要：目的:观察功能性便秘、腹泻模型大鼠体表敏化区的分布特点,探究不同强度电针“天枢”对大鼠远端结肠运动的调节规律,明确肠神经系统(ENS)内不同亚型神经元在该调节效应中发挥的作用。方法:选用90只SPF级雄性SD大鼠,将其中15只大鼠随机分为正常组、便秘组、腹泻组,每组5只,观察粪便形态与含水量,以及尾静脉注射伊文氏蓝(EB)后大鼠体表EB渗出点的分布规律;18只大鼠随机分为正常+2 mA组、正常+4 mA组和正常+6 mA组,每组6只,利用生理信号采集分析系统记录、分析大鼠结肠蠕动波曲线下面积和平均幅值,观察不同强度电针“天枢”对远端结肠蠕动的即刻效应;30只大鼠随机分为正常组、便秘组、腹泻组、便秘+2 mA组、腹泻+6 mA组,每组6只,观察电针“天枢”对便秘和腹泻模型大鼠结肠动力紊乱的累积效应;12只大鼠随机分为便秘+2 mA组、腹泻+6 mA组,每组6只,用于观察电针“天枢”对便秘和腹泻模型大鼠结肠动力紊乱的即刻效应;15只大鼠随机分为正常组、便秘组、腹泻组、便秘+2 mA组、腹泻+6 mA组,每组3只,采用whole-mount染色检测ENS内囊泡乙酰胆碱转运体(VAChT)阳性神经元和一氧化氮合酶(n NOS)阳性神经元表达情况。根据分组,通过盐酸洛哌丁胺灌胃(10 mg·kg^(-1)·d^(-1),连续7 d)建立功能性便秘模型,番泻叶药液灌胃(10 mL/kg,每天1次,连续2 d)建立功能性腹泻模型;于双侧“天枢”行不同强度(电流2、4、6 mA)电针干预,连续波,频率10 Hz。结果:与正常组比较,便秘组大鼠粪便数量减少,粪便含水量降低(P<0.001);腹泻组大鼠出现便溏症状,粪便含水量升高(P<0.001)。便秘组和腹泻组大鼠体表EB渗出点可见于T6-S2节段区域,且在T10-L3节段的下腹部和下背部较为集中。与电针前比较,正常+2 mA组、正常+4 mA组大鼠电针过程中远端结肠蠕动波曲线下面积和平均幅值升高(P<0.01,P<0.05),表现为促�Objective To observe the distribution characteristics of sensitization areas on the body surface in the rat models with functional constipation and diarrhea,explore the regulatory patterns of electroacupuncture(EA)of different intensities at"Tianshu"(ST25)on distal colon motility,and clarify the roles of the neurons of different subtypes in the enteric nervous system(ENS)displayed in the regulatory effect.MethodsOf 90 SD male rats of SPF grade,15 rats were randomized into a normal group,a constipation group and a diarrhea group,5 rats in each one.The stool form and fecal water content,as well as the distribution of the Evans blue(EB)extravasation on the body surface after the intravenous injection with EB on the tails were observed.Eighteen rats were randomized into a normal+2 mA group,a normal+4 mA group and a normal+6 mA group,6 rats in each one.Using physiological signal acquisition system,the area under the curve and the average amplitude of colon peristalsis were recorded and analyzed,and the immediate effect on distal colon peristalsis observed after EA with different intensities at"Tianshu"(ST25).Thirty rats were randomized into a normal group,a constipation group,a diarrhea group,a constipation+2 mA group,and a diarrhea+6 mA group,6 rats in each one,so as to observe the cumulative effect on colon motility disorder in the rat models of constipation and diarrhea after EA at"Tianshu"(ST25).Twelve rats were randomized into a constipation+2 mA group and a diarrhea+6 mA group,6 rats in each one,to observe the immediate effect on colon motility disorder in the rat models of constipation and diarrhea after EA at"Tianshu"(ST25).Fifteen rats were randomly divided into a normal group,a constipation group,a diarrhea group,a constipation+2 mA group,and a diarrhea+6 mA group,3 rats in each one.Using the whole-mount staining technique,the expression of vesicular acetylcholine transporter(VAChT)-positive neurons and nitric oxide synthase(nNOS)-positive neurons in ENS was detected.According to the group divisions,the func

关 键 词：功能性肠病 电针 天枢 结肠运动 双向调节 肠神经系统 

分 类 号：R245[医药卫生—针灸推拿学]