直立百部块根中酚类化学成分与抗炎活性研究  

Phenolic constituents isolated from Stemona sessilifolia roots with their antiinflammatory activities

作　　者：赵超越 谷文宇 王文君 贾献慧 白著双 唐文照 ZHAO Chaoyue;GU Wenyu;WANG Wenjun;JIA Xianhui;BAI Zhushuang;TANG Wenzhao(School of Pharmaceutical Sciences&Institute of Materia Medica,Shandong First Medical University&Shandong Academy of Medical Sciences,National Key Laboratory of Advanced Drug Delivery System,Key Laboratory for Biotechnology Drugs of National Health Commission(Shandong Academy of Medical Sciences),Key Lab for Rare&Uncommon Diseases of Shandong Province,Jinan 250117,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)药学院(药物研究所)、先进药物递释系统全国重点实验室、国家卫健委生物技术药物重点实验室、山东省罕少见病重点实验室,山东济南250117

出　　处：《中草药》2025年第5期1519-1524,共6页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局科技司-山东省卫生健康委员会共建中医药科技项目(GZY-KJS-SD-2023-087)。

摘　　要：目的对直立百部Stemona sessilifolia块根的化学成分进行分离鉴定。方法采用硅胶、Sephadex LH-20、ODS等柱色谱及制备型高效液相等方法进行分离纯化;根据化合物的波谱数据(MS、1H-与13C-NMR)鉴定其结构;利用CCK-8法及Griess法测定化合物的细胞毒活性及抗炎活性(NO含量)。结果从直立百部块根的乙醇提取物中分离得到8个酚类化合物,分别鉴定为4-氯-3-甲氧基-5-甲基苯酚-1-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷(1)、4-氯-3-甲氧基-5-甲基苯基-1-O-α-L-吡喃鼠李糖基-(1→6)-β-D-吡喃葡萄糖苷(2)、3,4,5-三甲氧基苯基-1-O-β-D-吡喃葡萄糖苷(3)、刺五加苷B(4)、肿柄雪莲苷(5)、(+)-异落叶松树脂醇-3α-O-β-D-吡喃葡萄糖苷(6)、去氢-δ-生育酚(7)与去氢-γ-生育酚(8)。结论化合物1为1个新化合物,化合物2~6为首次从百部属植物中分离得到,7和8为首次从直立百部中分离得到;化合物3、4、7和8对RAW 264.7细胞没有明显的细胞毒性,存活率均大于85%,其具有弱的抗炎活性,在20μmol/L下对脂多糖诱导的RAW 264.7细胞炎症因子NO释放的抑制率在20%~50%。Objective To study chemical constituents in the Stemona sessilifolia roots with their anti-inflammatory activities.Methods Compounds were isolated and purified by various chromatography,including silica gel,ODS,Sephadex LH-20 and preparative HPLC.Their structures were elucidated by spectroscopic data analysis.Cytotoxicity and anti-inflammatory activities were evaluated by CCK8 and Griess experiments.Results Eight compounds were obtained from the tuber roots of S.sessilifolias and identified as 4-chloro3-methoxy-5-methylphenol-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(1),4-chloro-3-methoxy-5-methylphenyl-1-O-α-Lrhanopyranosyl-(1→6)-β-D-glucopyranoside(2),3,4,5-trimethoxyphenyl-1-O-β-D-glucopyranoside(3),eleutheroside B(4),conicaoside(5),(+)-isolariciresinol-3α-O-β-D-glucopyranoside(6),dehydro-δ-tocopherol(7)and dehydro-γ-tocopherol(8).Conclusion Compound 1 is a new natural product,compounds 2―6 were isolated from Stemona genus for the first time,and compounds 7 and 8 were obtained firstly from S.sessilifolias.Compounds 3,4,7 and 8 showed no cytotoxicity on RAW 264.7 cells and weak anti-inflammatory activities all with 20%―50%NO inhibition at 20μmol/L.

关 键 词：直立百部 酚类化合物 细胞毒活性 抗炎活性 4-氯-3-甲氧基-5-甲基苯酚-1-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷 3 4 5-三甲氧基苯基-1-O-β-D-吡喃葡萄糖苷 刺五加苷B 肿柄雪莲苷 

分 类 号：R284.1[医药卫生—中药学]