胆南星多糖组分拮抗高热惊厥模型大鼠神经炎症的作用  

Antagonistic effect of Arisaema Cum Bile polysaccharides on neuroinflammation in febrile seizure model rats

作　　者：李青霞 苏发智 白晨曦 贾晨宸 孙延平[1] 匡海学[1] 王秋红[1,2] LI Qingxia;SU Fazhi;BAI Chenxi;JIA Chenchen;SHUN Yanping;KUANG Haixue;WANG Qiuhong(Heilongjiang Provincial Key Laboratory of Pharmacodynamic Material Basis of Chinese Medicine and Natural Drugs,Key Laboratory of Basic and Application Research of Beiyao,Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Department of Chinese Materia Medica Processing,School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]黑龙江中医药大学,教育部北药基础与应用研究重点实验室,黑龙江省中药天然药物药效物质基础重点实验室,黑龙江哈尔滨150040 [2]广东药科大学中药学院,中药炮制学教研室,广东广州510006

出　　处：《中草药》2025年第5期1628-1639,共12页Chinese Traditional and Herbal Drugs

基　　金：2022年全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号);第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号);黑龙江省“头雁”团队支持项目(黑龙江省头雁行动领导小组文件[2019]5号)。

摘　　要：目的研究胆南星Arisaema Cum Bile多糖组分拮抗高热惊厥模型大鼠神经炎症作用的可能机制。方法采用水提醇沉法制备胆南星多糖,并通过色谱、光谱等方法对其进行结构表征分析;通过ip脂多糖联合热水浴建立高热惊厥模型,随机分为对照组、模型组、丙戊酸钠(200 mg/kg)组和胆南星高、低剂量(200、100 mg/kg)组,每组10只。记录每组大鼠惊厥潜伏期,惊厥持续时间和惊厥级别,并于末次造模2 h后测量大鼠肛温;苏木素-伊红(hematoxylin eosin,HE)染色评估海马组织神经元病理变化;ELISA法检测血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、IL-6及海马组织中γ-氨基丁酸(γ-aminobutyricacid,GABA)、谷氨酸(glutamic acid,Glu)、NOD样受体热蛋白结构域3(NOD like receptor family pyrin domain containing 3,NLRP3)和高迁移率族蛋白B1(high mobility group box 1 protein,HMGB1)水平;采用超高效液相色谱-四级杆飞行时间质谱(ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry,UPLC-Q-TOF-MS/MS)对大鼠的血清进行代谢组学分析。结果分离制备得到的胆南星多糖中总糖、蛋白质和糖醛酸质量分数分别为79.4%、2.96%和2.35%;气相色谱-质谱联用技术(gas chromatographymass spectrometry,GC-MS)测定胆南星多糖主要由阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成,其物质的量比分别为0.030∶0.260∶0.115∶0.741∶0.088;傅里叶变换红外光谱仪(Fouriertransform infrared spectrometer,FT-IR)光谱表明多糖含有吡喃环;与模型组比较,胆南星多糖高剂量组大鼠肛温降低(P<0.01)且胆南星多糖高、低剂量组均可显著延长惊厥潜伏期(P<0.01),缩短惊厥持续时间(P<0.01);对海马神经元细胞具有保护作用,升高海马组织GABA含量,同时降低血清中TNF-α、IL-1β和IL-6与海马组织中Glu、NLRP3及HMGB1含量(P<0.05、0.01);代谢组学结果表明,胆�Objective To investigate the possible mechanisms by which polysaccharides of Dannanxing(Arisaema Cum Bile)inhibit neuroinflammation in febrile seizure rat model.Methods The polysaccharides of Arisaema Cum Bile were prepared by a combination of water extraction and alcohol precipitation,and its primary structure was characterized by chromatographic and spectral methods.Febrile seizure model was established by intraperitoneal injection of lipopolysaccharide combined with hot water bath.The rats were randomly divided into five groups,including control group,model group,sodium valproate group(200 mg/kg),Arisaema cum bile polysaccharides high-and low-dose(200,100 mg/kg)groups,with ten rats in each group.The convulsive latency,duration and grade of convulsion were recorded in each group,and the rectal temperature of rats was monitored 2 h after the last modeling.Hematoxylin eosin(HE)staining was used to evaluate the pathological changes of hippocampal neurons.The levels of tumor necrosis factor-α(TNFα),interleukin-1β(IL-1β),IL-6 in serum andγ-aminobutyricacid(GABA),glutamic acid(Glu),NOD like receptor family pyrin domain containing 3(NLRP3)and high mobility group box 1 protein(HMGB1)in hippocampus tissues were detected by ELISA.The metabolomics analysis of rat serum based on ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS)was performed.Results The total sugar,protein and glucuronic acid contents of the Arisaema Cum Bile polysaccharides obtained from the isolation preparation were 79.4%,2.96%and 2.35%,respectively.The results of monosaccharide composition based on gas chromatography-mass spectrometry(GC-MS)exhibited that the polysaccharides of Arisaema Cum Bile were mainly composed of arabinose,xylose,mannose,glucose and galactose with molar mass ratios of 0.030∶0.260∶0.115∶0.741∶0.088,respectively.Fouriertransform infrared spectrometer(FT-IR)spectroscopy indicated that the polysaccharides contained pyran ring.Compared with the model group,the rectal

关 键 词：胆南星 多糖组分 高热惊厥 神经炎症 代谢组学 解热 海马神经元 

分 类 号：R285.5[医药卫生—中药学]