茵陈总黄酮改善非酒精性脂肪肝病小鼠肝损伤的机制研究  

Mechanism of Artemisia capillaris total flavonoids in ameliorating liver injury in non-alcoholic fatty liver disease mice

作　　者：刘扬 张中华 张喜华 周超雄[1] 熊杰 何前进[1] LIU Yang;ZHANG Zhonghua;ZHANG Xihua;ZHOU Chaoxiong;XIONG Jie;HE Qianjin(Department of Hepatobiliary Surgery,Huanggang Center Hospital,Huanggang,Hubei 438000,China)

机构地区：[1]黄冈市中心医院肝胆外科,湖北黄冈438000

出　　处：《中国病原生物学杂志》2025年第5期582-586,596,共6页Journal of Pathogen Biology

摘　　要：目的探讨茵陈总黄酮对非酒精性脂肪肝病小鼠的改善作用及机制。方法对C57BL/6J小鼠采用高脂饮食12周的方法建立非酒精性脂肪肝小鼠模型,分为模型组、二甲双胍组[200 mg/(kg·d)]、茵陈总黄酮组[100 mg/(kg·d),200 mg/(kg·d)],每组8只,并选择8只野生型作空白对照。给药12周后,采集小鼠血清及肝组织,检测血脂指标总胆固醇、三酰甘油、高密度脂蛋白、低密度脂蛋白及肝功能指标谷丙转氨酶、谷草转氨酶;肝脏脂质指标总胆固醇、三酰甘油,脂质过氧化指标丙二醛及超氧化物歧化酶;测定血清炎症因子指标白介素6、肿瘤坏死因子;H&E染色法观察肝脏病理形态学变化;测定肝组织中PI3K、AKT、FASN、ACC、SREBP-1C的基因表达。结果与模型组相比,茵陈总黄酮能显著降低NAFLD小鼠的体重、血脂水平;并能改善肝脏功能、肝脏脂质蓄积、炎症及氧化应激水平,减轻肝脏脂肪肝病样变及炎症浸润,并显著调控PI3K/AKT信号通路上关键因子的表达,其中以茵陈总黄酮高剂量的改善效果最佳。结论茵陈总黄酮可能通过PI3K/AKT通路改善NAFLD小鼠的肝脏病变。Objective To investigate the effect and mechanism of total flavonoids of Yin-chen on mice with nonalcoholic fatty liver disease.Methods C57BL/6J mice were fed a high-fat diet for 12 weeks to establish a nonalcoholic fatty liver model,which was divided into four group containing model group,metformin group[200 mg/(kg·d)],total flavonoids group[100 mg/(kg·d),200 mg/(kg·d)],with 8 mice in each group.And 8 wild-type mice were selected as blank control.After 12 weeks of administration,serum and liver tissues of mice were collected.The serum total cholesterol(TC),triacylglycerol(TG),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),and glutamic-pyruvic transaminase(ALT)and glutamic-oxalacetic transaminase(AST)were detected.Liver total cholesterol and triglyceride were detected,while lipid peroxidation index including malondialdehyde and superoxide dismutase were measured.Serum interleukin-6(IL-6)and tumor necrosis factor(TNF-α)were tested.The pathological changes of liver were observed by H&E staining.The gene expression of PI3K,AKT,FASN,ACC and SREBP-1C in liver tissue was determined.Results Compared with the model group,total flavonoids of Yin-chen could significantly reduce the body weight and the levels of blood lipid,including TC,TG,LDL-C in NAFLD mice.Moreover,it can improve the indexes of liver function including ALT and AST,liver lipid accumulation,inflammation and oxidative stress levels,and significantly regulate the expression of key factors in PI3K/AKT signaling pathway,among which high dose of total flavonoids has the best effect.Conclusion The total flavonoids of Yin-chen may improve the liver lesions of NAFLD mice through PI3K/AKT pathway.

关 键 词：茵陈 黄酮 非酒精性脂肪肝 PI3K-AKT信号通路 

分 类 号：R285.5[医药卫生—中药学]