桦褐孔菌多糖对新孢子虫感染雄鼠主要脏器组织和激素的影响  

Effects of Inonotus obliquus polysaccharides on main organs and hormones in male mice infected with Neospora caninum

作　　者：刘萌 林思呈 赵芳琳 王振宇 王洋[1] 闵鹏飞 李璐 贾立军[1] LIU Meng;LIN Sicheng;ZHAO Fanglin;WANG Zhenyu;WANG Yang;MIN Pengfei;LI Lu;JIA Lijun(Engineering Research Center of Science and Technology Innovation of Beef Cattle in Northeast Cold Region,Ministry of Education,Agricultural College of Yanbian University,Yanbian,Jilin133000,China)

机构地区：[1]延边大学农学院东北寒区肉牛科技创新教育部工程研究中心,吉林延吉133000

出　　处：《中国兽医学报》2025年第2期249-254,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32360885,32160839);吉林省教育厅“十四五”科学技术资助项目(JJKH20230633KJ);高等学校学科创新引智111计划资助项目(D20034);2024年大学生创新训练计划资助项目。

摘　　要：为确定桦褐孔菌多糖对新孢子虫感染雄鼠主要脏器组织和激素的影响,建立了新孢子虫病BALB/c小鼠动物模型,灌胃给药后,病理组织学观察小鼠脑、肝脏、脾脏组织病理变化,qPCR检测小鼠脑、肝脏、脾脏组织中所含的虫荷量,ELISA检测雄性小鼠血清中FSH、LH、T4激素水平。结果显示,与正常对照组比较,模型组小鼠脑组织可见颗粒状的球形病灶,肝脏细胞呈片状坏死,脾脏组织出血、红髓充血、可见大量红细胞浸润,而桦褐孔菌多糖能够改善脑、肝脏、脾脏等器官组织的损伤。桦褐孔菌多糖组小鼠脑中虫荷量在14和21 d时均极显著低于模型组(P<0.01),肝脏、脾脏中虫荷量在21 d时均极显著低于模型组(P<0.01);FSH水平模型组和桦褐孔菌多糖组均显著低于正常对照组(P<0.05或P<0.01),而桦褐孔菌多糖组均显著高于模型组(P<0.05或P<0.01);LH水平模型组均极显著高于正常对照组(P<0.01),而桦褐孔菌多糖组在7和42 d时趋于正常对照组但差异显著(P<0.05);T4水平模型组与空白组比较差异显著(P<0.05或P<0.01),而桦褐孔菌多糖组在21和35 d时趋于正常对照组(P>0.05)。桦褐孔菌多糖能够改善新孢子虫对雄鼠脑、肝脏、脾脏组织的损伤作用,降低脑、肝脏、脾脏中虫荷量,调节FSH、LH、T4激素水平,维持生殖系统稳定。The aim of this study is to determine the effects of Inonotus obliquus polysaccharides on the main organs and hormones of male mice infected with Neosporidium.The animal model of neosporidiosis in BALB/c mice was established.After intragastric administration,the pathological changes of brain,liver and spleen were observed by histopathology,the worm load in brain,liver and spleen was detected by qPCR,and the levels of FSH,LH and T4 in serum of male mice were detected by ELISA.The results showed that compared with the blank control group,granular spherical lesions could be seen in the brain tissue of mice in the model group,with patchy necrosis of hepatocytes,splenic hemorrhage,red pulp hyperemia and a large number of red blood cell infil-tration.Inonotus obliquus polysaccharides can improve the injury of brain,liver,spleen and other organs and tissues.The worm load in the brain of the Inonotus obliquus polysaccharide group was significantly lower than that of the model group on the 14th day and 21st day(P<0.01),and that of the liver and spleen on the 21st day was significantly lower than that of the model group(P<0.01).The level of FSH in the model group and Inonotus obliquus polysaccharide group was signif-icantly lower than that in the blank control group(P<0.05 or P<0.01),while that in the Inono-tus obliquus polysaccharide group was significantly higher than that in the model group(P<0.05 or P<0.01).The level of LH in the model group was significantly higher than that in the blank control group(P<0.01),while that in the Inonotus obliquus polysaccharide group was significant-ly higher than that in the blank control group on the 7th day and 42nd day(P<0.05).There was significant difference in T4 level between the model group and the blank group(P<0.05 or P<0.01),while the Inonotus obliquus polysaccharide group was similar to the blank control group on the 21st and 35th day(P>0.05).Inonotus obliquus polysaccharides can improve the damage of Neosporidium on brain,liver and spleen of male rats,reduce the insect load

关 键 词：桦褐孔菌多糖 新孢子虫病 脏器 病理 激素 小鼠 

分 类 号：S859.7[农业科学—临床兽医学]