脱氧胆酸(DCA)对金黄色葡萄球菌诱导小鼠乳腺炎的治疗作用及机制  

Protective effect and mechanism of deoxycholic acid(DCA)on Staphylococcus aureus induced mastitis in mice

作　　者：董子婷 梁超慧 孙青松 张月 李沐森 DONG Ziting;LIANG Chaohui;SUN Qingsong;ZHANG Yue;LI Musen(College of Animal Science and Technology,Jilin University of Agricultural Science and Technology,Jilin City,Jilin132109,China)

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林132109

出　　处：《中国兽医学报》2025年第2期274-280,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32301247)。

摘　　要：乳腺炎作为危害奶业发展的重要疾病,其发生给养殖业造成巨大经济损失。金黄色葡萄球菌(Staphylococcus aureus,S.aureus)是导致奶牛乳腺炎的重要致病菌之一。先前已有研究发现脱氧胆酸(deoxycholic acid, DCA)在许多疾病中发挥重要作用,但其对乳腺炎的作用尚未有过报道。为了探究DCA对S.aureus诱导小鼠乳腺炎的作用及机制,本研究构建了S.aureus诱导的小鼠乳腺炎模型,腹腔注射DCA(0.01、0.02、0.03 mg/g),收集乳腺组织,检测DCA对乳腺炎的保护作用及机制。结果发现,DCA可以呈剂量依赖的方式有效减轻S.aureus引发的小鼠乳腺组织病理学损害程度;同时,它能够抑制由S.aureus诱导产生的炎症介质,包括肿瘤坏死因子TNF-α、白细胞介素IL-1β的表达水平,以及髓过氧化物酶MPO的活性;DCA可降低MDA和铁离子浓度,同时上调GSH和GPX4表达,表明DCA可抑制S.aureus诱导的铁死亡;Western blot结果显示,DCA可上调TGR5受体,进而抑制NF-κB信号通路。以上结果表明,DCA通过调控TGR5-NF-κB信号通路抑制S.aureus诱导的炎症反应和铁死亡,发挥抗乳腺炎作用。Mastitis,as an important disease that endangers the development of the dairy industry,causes huge economic losses to the breeding industry.Staphylococcus aureus is one of the impor-tant pathogenic bacteria that cause mastitis in dairy cows.Previous studies have found that deoxy-cholic acid(DCA)plays an important role in many diseases,but its effect on mastitis has not been reported yet.In order to investigate the effect and mechanism of DCA on Staphylococcus aureus in-duced mouse mastitis,this study constructed a Staphylococcus aureus induced mouse mastitis model,mice were intraperitoneally injected DCA(0.01,0.02,0.03 mg/g),breast tissue were col-lected,and the protective effect and mechanism of DCA on mastitis were evalued.The results showed that DCA dose dependently reduced the pathological damage of mouse breast tissue in-duced by Staphylococcus aureus;DCA inhibited the expression of inflammatory cytokines TNF-αand IL-1βand MPO activity induced by Staphylococcus aureus;DCA significantly reduced the con-centration of MDA and iron ions,while upregulating the expression of GSH and GPX4,indicating that DCA could inhibit ferroptosis induced by Staphylococcus aureus;Western blot results showed that DCA could upregulate TGR5 receptors,thereby inhibiting the NF-κB signaling pathway.The above results indicate that DCA inhibits mastitis by regulating the TGR5-NF-κB signaling pathway to inhibit the inflammatory response and ferroptosis induced by Staphylococcus aureus.

关 键 词：脱氧胆酸 乳腺炎 TGR5受体 炎性细胞因子 NF-κB 小鼠 

分 类 号：S854.5[农业科学—临床兽医学]