猪源奇异变形杆菌感染胃肠道后小鼠血清中细胞因子的动态变化  

Dynamic changes of serum cytokines in mice following gastrointestinal infection with porcine-derived Proteus mirabilis

作　　者：周杰 付文贵[1,2,3,4] 郝成森 李绍梅 翟少钦 杨睿[1,2,3,4] ZHOU Jie;FU Wengui;HAO Chengsen;LI Shaomei;ZHAI Shaoqin;YANG Rui(Chongqing Academy of Animal Science,Rongchang,Chongqing 402460,China;National Center of Technology Innovation for Pigs,Rongchang,Chongqing 402460,China;National Animal Disease-Chongqing Monitoring Station,Rongchang,Chongqing 402460,China;Chongqing Research Center of Veterinary Biological Products Engineering Technology,Rongchang,Chongqing 402460,China;College of Veterinary Medicine,Southwest University,Rongchang,Chongqing 402460,China)

机构地区：[1]重庆市畜牧科学院,重庆荣昌402460 [2]国家生猪技术创新中心(重庆),重庆荣昌402460 [3]国家动物疫病重庆观测实验站,重庆荣昌402460 [4]重庆市兽用生物制品工程技术中心,重庆荣昌402460 [5]西南大学动物医学院,重庆荣昌402460

出　　处：《中国兽医学报》2025年第2期281-288,共8页Chinese Journal of Veterinary Science

基　　金：重庆市自然科学基金面上资助项目(cstc2021jcyj-msxmX0456);四川省区域创新合作资助项目(2023YFQ0037)。

摘　　要：奇异变形杆菌(Proteus mirabilis,PM)是一种重要的食源性病原,经常导致人和动物发病,因此了解PM感染胃肠道后机体中细胞因子的变化情况对于制定有效的治疗措施具有重要意义。此项研究采用灌胃方式对小鼠进行攻毒,观察小鼠的临床症状和病理学变化,分别在感染后不同时间段采集小鼠血液,利用Bio-Plex悬液芯片检测系统对攻毒和对照组小鼠血清中的21种细胞因子进行定量检测和分析。攻毒结果显示,PM感染小鼠的胃肠道后,可导致小鼠的脾脏出血并显著肿大,在攻毒后24 h出现显著性差异(P=0.001 688),攻毒后96 h 2组小鼠间的脾指数差异最大(P=0.000 074)。细胞因子的检测结果显示,IL-2、IL-17A、KC、Eoatxin、MCP-1、RANTES、MIP-1α、MIP-1β、IFN-γ、G-CSF和IL-10的浓度在攻毒组中显著性增高,其中促炎因子IL-17A和G-CSF的含量变化最大,并与脾脏指数的变化趋势相同,均在攻毒后96 h达到峰值,质量浓度分别为(104.74±3.91)和(5 184.08±280.22) ng/L,是对照组的26.79和36.25倍。表明在PM感染胃肠道后,IL-17A和G-CSF在机体的促炎反应中发挥着重要的作用。Proteus mirabilis is a significant foodborne pathogen that frequently causes diseases in humans and animals.Understanding the cytokine profile alterations following gastrointestinal in-fection with P.mirabilis is crucial for developing effective treatment strategies.This study em-ployed an intragastric infection model in mice to observe clinical symptoms and pathological chan-ges.Blood samples were collected at various time points post-infection,and the concentrations of 21 cytokines in the serum of both infected and control mice were quantitatively analyzed using the Bio-Plex suspension array system.The results of the infection experiment indicated that gastroin-testinal infection with P.mirabilis in mice led to splenic hemorrhage and significant splenomegaly.A notable difference in spleen index between the infected and control groups was observed at 24 hours post-infection(P=0.001688),with the greatest difference occurring at 96 hours post-infec-tion(P=0.000074).Cytokine analysis revealed significant elevations in IL-2,IL-17A,KC,Eotax-in,MCP-1,RANTES,MIP-1α,MIP-1β,IFN-γ,G-CSF,and IL-10 levels in the infected group.A-mong these,the pro-inflammatory cytokines IL-17A and G-CSF exhibited the most substantial changes,positively correlating with the splenomegaly,and peaking at 96 hours post-infection,with concentrations of(104.74±3.91)and(5184.08±280.22)ng/L,respectively,which were 26.79 and 36.25 times higher than those in the control group.These findings indicate that IL-17A and G-CSF play significant roles in the pro-inflammatory response following gastrointestinal infection with P.mirabilis.

关 键 词：奇异变形杆菌 细胞因子 脾脏 小鼠 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]