从河南省猪群中鉴定出PRRSV1病毒感染  

Identification of porcine reproductive and respiratory syndrome infection in pig herds from Henan Province

作　　者：王新港 郭占达 杜季梅 姬星宇 张先锋 陈陆[3] 王彦辉 王川庆[3] WANG Xingang;GUO Zhanda;DU Jimei;JI Xingyu;ZHANG Xianfeng;CHEN Lu;WANG Yanhui;WANG Chuanqing(Shangqiu Meilan Bioengineering Co.,Ltd.,Shangqiu,Henan 476200,China;Henan Dahe Agriculture and Animal Husbandry Technology Co.,Ltd.,Zhengzhou 450002,China;Laboratory for Infectious Diseases,College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]商丘美兰生物工程有限公司,河南商丘476200 [2]河南省大河农畜牧科技有限公司,河南郑州450002 [3]河南农业大学动物医学院传染病实验室,河南郑州450002

出　　处：《中国兽医学报》2025年第3期427-435,共9页Chinese Journal of Veterinary Science

基　　金：中原科技创新领军人才资助项目(244200510006)。

摘　　要：为了解猪繁殖与呼吸综合征病毒种1(porcine reproductive and respiratory syndrome virus 1,PRRSV1)在河南省的流行情况,本研究对河南省部分规模化猪场临床病料进行分子流行病学调查与病毒分离鉴定。利用PRRSV1 ORF5基因特异性引物进行RT-PCR检测与序列测定,结果共检测出PRRSV1阳性样品8份,获得ORF5基因序列6个,全基因序列1个。利用PAM细胞成功分离出1株PRRSV1,命名为HENZMD-10。对分离毒株基因遗传变异分析,获得的6个ORF5基因均为PRRSV1,分离毒株ORF5基因遗传变异较大,遗传进化树上分属于不同的分支。其中,HENJZ-11、HENJY-7、HENJY-8遗传进化距离较近,分属于同一分支。HENZMD-10分离株基因组全长为15071 bp,核苷酸序列比对与LV毒株核苷酸相似性为89.1%;与PRRSV2 ATCC-VR2332毒株核苷酸相似性为62.1%;与美国NADC30毒株核苷酸相似性为61.5%;与国内JXA1毒株核苷酸相似性为61.6%。全基因组遗传进化分析显示,HENZMD-10与国内分离PRRSV1毒株NVDC-NM1-2011、LNEU12和FJEU13毒株的遗传距离较近。结果表明,本试验通过对河南省PRRSV1的分子流行病学调查以及病毒全基因遗传进化分析,成功分离1株PRRSV1,为PRRSV1在国内的流行动态及其防控提供一定的临床指导意义。In order to understand the epidemic situation of European porcine reproductive and respiratory syndrome virus 1(PRRSV1)in Henan Province,molecular epidemiological investigation and virus identification were conducted on clinical specimens from some large-scale pig farms in Henan Province.RT-PCR detection and sequencing were carried out using specific primers for PRRSV1 ORF5 gene.As a result,eight PRRSV1 positive samples were detected with six ORF5gene sequences and one full gene sequence obtained.A strain of PRRSV1 named as HENZMD-10was successfully isolated using PAM cells.Based on the genetic variation analysis,the six ORF5genes obtained were all PRRSVl.The isolated strains have relatively large ORF5 gene genetic variation,belonging to different branches on the genetic evolution tree.Among them,HENJZ-11,HENJY-7,and HENJY-8 were relatively close in genetic evolution and belonged to the same branch.The genome length of HENZMD-10 isolate was 15071 bp.Alignment analysis showed that HENZMD-10 strain shared an 89.1%of nucleotide sequence with the LV strain;62.1%and61.5%with PRRSV2 ATCC-VR2332 strain and the American NADC30 strain,respectively.The nucleotide similarity of HENZMD-10 strain with the domestic JXA1 strain was 61.6%.The phylogenetic analysis using complete genome sequence showed that HENZMD-10 strain was relatively close to the domestic isolated PRRSV1 NVDC-NM1-2011,LNEU12 and FJEU13 strains.The successful isolation of one PRRSV1 strain in Henan Province provides the basis for understanding the epidemic dynamics and prevention and control of PRRSV1 in China.

关 键 词：PRRSV PRRSV1 ORF5基因 全基因测序 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]