中国猫博卡病毒病的流行病学调查及遗传变异分析  

Epidemiological investigation and geneticvariation of feline bocavirus disease in China

作　　者：李勇璠 李尉晖 颜权辉 杜雯欣 曹龙龙 李佳康 曾悦 曹胜波[1] 李秋燕 周登元 LI Yongfan;LI Weihui;YAN Quanhui;DU Wenxin;CAO Longlong;LI Jiakang;ZENG Yue;CAO Shengbo;LI Qiuyan;ZHOU Dengyuan(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Wuhan Keqian Biology Co.,Ltd.,Wuhan 430040,China;College of Animal Science and Technology,Henan Institute of Science and Technology,Xinxiang,Henan 453003,China)

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]武汉科前生物股份有限公司,湖北武汉430040 [3]河南科技学院动物科技学院,河南新乡453003

出　　处：《中国兽医学报》2025年第3期489-497,共9页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2023YFD1800402);湖北省试验动物研究资助项目(2023CFA005)。

摘　　要：2022年12月至2023年12月期间,在全国采集2560份拭子样品,利用PCR方法对猫博卡病毒(feline bocavirus,FBoV)进行检测并扩增其NS1及VP2基因编码序列;同时,利用生物信息学方法分析FBoV的遗传多样性。结果显示,FBoV的总阳性率为4.6%(119/2560),共在8个省或直辖市的样品中检出FBoV,疾病呈区域性流行特点;基因遗传进化分析结果显示,FBoV以多种基因型存在,我国主要以FBoV-1型流行为主,本研究鉴定的15株FBoV-1型毒株、4株FBoV-2型毒株、1株FBoV-3型毒株与中国、美国、泰国、澳大利亚、葡萄牙所鉴定的毒株遗传距离较近;同源性分析结果显示,各测序毒株与参考毒株间NS1及VP2基因氨基酸同源性分别为60.40%~99.20%和67.20%~100%,各测序毒株之间NS1及VP2氨基酸同源性分别为60.60%~100%和67.80%~100%,结果表明,我国流行的FBoV毒株具有明显的遗传多样性。本研究为阐明我国FBoV的流行现状充实了资料,并为后续针对FBoV的诊断或防控提供了流行病学调查基础。Two thousand five hundred and sixty swab samples were collected from December 2022to December 2023 in China.PCR was used to detect FBoV and amplify its VP2 and NS1 gene coding sequences,and bioinformatics was used to analyze the genetic diversity of FBoV.The results showed that the total positive rate of FBoV was 4.6%(119/2560).Genetic variation analysis showed that FBoV existed in a variety of genotypes,and FBOV-1 was the main epidemic type in China.The 15 FBoV-1 strains,four FBoV-2 strains and one FBoV-3 strains identified in this study were genetically close to the strains identified in China,the United States,Thailand,Australia and Portugal.Sequence analysis showed that the identities of amino acid sequence of NS1 and VP2genes between the sequenced strains and the reference strains were 59.13%-99.25%and 96.41%-100.00%,respectively.The amino acid identities of NS1 and VP2 among the newly sequenced FBoV strains were 60.00%-100.00%and 96.41%-100.00%,respectively,which indicated that the FBov strains circulating in China had great genetic diversity.This study enriched the data for elucidating the epidemic status of FBoV in China,and provided the basis for the subsequent diagnosis,prevention and control of FBoV.

关 键 词：猫博卡病毒 流行病学调查 遗传进化分析 基因分型 

分 类 号：S852.65[农业科学—基础兽医学]