BALB/c小鼠肠道主要原虫感染情况的分子流行病学调查  

Molecular epidemiological investigation of main intestinalprotozoa infection in BALB/c mice

作　　者：郑瑜 张西臣 荣尧 李建华 宫鹏涛 王晓岑 岳涛涛 李新 张旭 张楠 ZHENG Yu;ZHANG Xichen;RONG Yao;LI Jianhua;GONG Pengtao;WANG Xiaocen;YUE Taotao;LI Xin;ZHANG Xu;ZHANG Nan(State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of Ministry of Education,College of Veterinary Medicine,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物医学学院人兽共患病研究教育部重点实验室/人畜共患传染病重症诊治全国重点实验室,吉林长春130062

出　　处：《中国兽医学报》2025年第3期514-518,共5页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2021YFF0702900,2023YFD1801000);国家自然科学基金资助项目(32102696)。

摘　　要：分别对国内4家BALB/c小鼠供应商提供的540只BALB/c小鼠进行肠道原虫检测。其中,供应商A 140只,供应商B 130只,供应商C 135只,供应商D 135只。分别采集每只小鼠的新鲜粪便样本以提取基因组,并根据人五毛滴虫(Pentatrichomonas hominis,P.hominis)、泰泽隐孢子虫18S rRNA基因序列引物进行巢氏PCR扩增,利用鼠三毛滴虫(Tritrichomonas muris,T.muris)16S-like rRNA基因序列引物进行PCR扩增并测序,以检测BALB/c小鼠肠道原虫的感染状况。结果显示,供应商A提供的140份小鼠粪便样本中,总肠道原虫感染率为7.1%(10/140),其中,T.muris虫阳性率为7.1%(10/140)、泰泽隐孢子虫(Cryptosporidium tyzzeri,C.tyzzeri)阳性率为2.1%(3/140)、P.hominis阳性率为7.1%(10/140),2种肠道原虫共感染率为7.1%(10只:T.muris+P.hominis)、3种肠道原虫共感染率为2.1%(3只:T.muris+P.hominis+C.tyzzeri)。供应商C提供的135份小鼠粪便样本中,总肠道原虫感染率为7.4%,其中,T.muris阳性率为7.4%(10/135),未检出C.tyzzeri和P.hominis感染。供应商B提供的130份小鼠粪便样本和供应商D提供的135份小鼠粪便样本中均未检出肠道原虫感染。遗传进化分析结果显示,本研究扩增的T.muris 16S-like rRNA基因序列与GenBank发表的T.muris的16S-like rRNA基因具有98.52%的同源性(GenBank No:AY886846.1),并且在遗传进化树中聚类为一独立分支;P.hominis 18S rRNA基因序列与GenBank发表的P.hominis 18S rRNA基因具有98.27%的同源性(GenBank No.AF156964.1),聚为一独立分支;C.tyzzeri 18S rRNA基因序列与GenBank发表的C.tyzzeri 18S rRNA基因同源性为99.87%(GenBank No.KJ000486.1)。综上,本研究对不同实验动物供应商提供的BALB/c小鼠进行肠道寄生虫检测,发现存在不同程度的肠道原虫感染情况,并发现有T.muris、P.hominis和C.tyzzeri等3种以上寄生虫共感染的现象,从而为今后预防和制定有效的防控策略提供科学依据。To understand the infection status of main intestinal protozoa in BALB/c mice and provide a basis for further control of intestinal protozoa infection.Five hundred and forty BALB/c mice provided by four domestic suppliers of BALB/c mice were detected for intestinal protozoa,in which 140 from supplier A,130 from supplier B,135 from supplier C,and 135 from supplier D,respectively.Fresh faecal samples were collected from each mouse separately to extract the genome and amplified by nested PCR based on primers for the 18S rRNA gene sequences of Pentatrichomonas hominis(P.hominis)and Cryptosporidium tyzzeri(C.tyzzeri),and the 16S-like rRNA gene sequence of Tritrichomonas muris(T.muris)and sequenced.The results showed that the total intestinal protozoan infection rate was 7.1%(10/140)in 140 mice faecal samples provided by supplier A.Among them,the positivity rate of T.muris was 7.1%(10/140),C.tyzzeri was2.1%(3/140),and P.hominis was 7.1%(10/140),the co-infection rate of two intestinal protozoa was 7.1%(10 mice:T.muris+P.hominis),and three intestinal protozoa was 2.1%(3 mice:T.muris+P.hominis+C.tyzzeri).The total intestinal protozoan infection rate in 135 mice faecal samples provided by supplier C was 7.4%,in which,7.4%(10/135)was positive for T.muris.There are no intestinal protozoa to be detected in 130 mice faecal samples from supplier B and 135mice faecal samples from supplier D.The homology analysis showed that the homology of amplified sequence of T.muris,P.hominis and C.tyzzeri was 98.52%,98.27%and 99.87%compared with published sequence of GenBank No:AY886846.1,GenBank No:AF156964.1 and GenBank No:KJ000486.1,which was clustered as an independent branch by phylogenetic analysis respectively.In conclusion,there are intestinal protozoan infection in BALB/c mice in some animal suppliers.The co-infections of more than 3 parasites such as T.muris,P.hominis and C.tyzzeri has been found.It will provide a basis for control of intestinal protozoa infection in BALB/c mice in the future.

关 键 词：鼠三毛滴虫 人五毛滴虫 泰泽隐孢子虫 BALB/c小鼠 

分 类 号：S855.9[农业科学—临床兽医学]