白头翁汤对湿热泄泻大鼠海马区5-HT信号系统的影响  

Effects of Pulsatilla decoction on 5-HT signaling system in the hippocampus of rats with damp-heat diarrhea

作　　者：曲韵琦 姜盛铭 冯硕 王晨颖 赖思越 马琪 QU Yunqi;JIANG Shengming;FENG Shuo;WANG Chenying;LAI Siyue;MA Qi(School of Animal Medicine,Southwest University,Chongqing 402460,China)

机构地区：[1]西南大学动物医学院,重庆荣昌402460

出　　处：《中国兽医学报》2025年第3期559-567,共9页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32202849);重庆市大学生创新创业训练计划资助项目(S202410635244)。

摘　　要：将24只雄性SD大鼠随机分为4组,分别为空白组、模型组、白头翁汤(Pulsatilla decoction,PD)组和自愈组。除空白组外,其余各组大鼠采用“高糖高脂+高温高湿+大肠杆菌攻毒”诱导建立湿热泄泻(damp-heat diarrhea,DHD)大鼠模型,并以白头翁汤灌胃治疗。通过记录观察大鼠体质量、体温及采食饮水量变化,检测血常规指标,观察结肠组织病理学变化,综合判定DHD大鼠造模情况;通过观察大鼠海马区组织病理学变化,采用实时荧光定量PCR测定海马区IL-1β、IL-6、TNF-α、IFN-γ和TPH1 mRNA表达量,利用Western blot和免疫组化检测海马区中5-HT信号通路色氨酸羟化酶1(TPH1)、受体(5-HT3R、5-HT4R、5-HT7R)的蛋白表达情况。结果显示:模型组大鼠PD大鼠体质量、采食饮水量下降,血常规指标异常变化,结肠组织损伤,PD可回调异常变化,改善结肠组织病理损伤;PD显著降低DHD大鼠海马区炎症细胞因子IL-1β、IL-6、IFN-γ、TNF-αmRNA表达量(P<0.05),降低DHD大鼠海马区TPH1mRNA表达量(P<0.05);PD能升高5-HT4R和5-HT7R在DHD大鼠脑海马区的表达;降低5-HT3R、TPH在脑海马区的表达,其中5-HT3R表达降低显著。结果表明,PD可通过调节5-HT信号通路影响DHD大鼠海马区功能。This study aims to investigate the effects of Pulsatilla decoction(PD)on the 5-HT signaling system in the hippocampus of damp-heat diarrhoea(DHD)rats.Twenty-four male SD rats were randomly divided into four groups including the blank group,the model group,the PD group and the self-healing group.Except for the blank group,the rats in each group were induced by"high sugar and high fat,high temperature and high humidity,and E.coli poisoning"to establish a of rat model DHD,and were treated by gavage with PD.Changes in body weight,temperature,food intake and water intake,routine blood tests and histopathological changes in the colon were recorded to comprehensively determine the modelling condition of rats with DHD;histopathological changes in the hippocampus of rats were observed,and real-time fluorescence PCR was used to determine the expression of IL-1β,IL-6,TNF-α,IFN-γand TPH1 mRNA in the hippocampus;Western blot and immunohistochemistry were used to detect the protein expression of tryptophan hydroxylase 1(TPH1),receptors(5-HT3R,5-HT4R,5-HT7R)in 5-HT signaling pathway in the hippocampus.The results showed that:PD significantly regulated the abnormal changes of body weight,food and water intake and blood routine indexes in rats with DHD,and significantly improved the pathological damage of colonic tissues;PD significantly lowered the expression of inflammatory cytokines IL-1β,IL-6,IFN-γ,and TNF-αin hippocampus of rats with DHD(P<0.05),and significantly reduced the expression of TPH1 mRNA in hippocampus of rats with DHD(P<0.05).PD could increased the expression of 5-HT4R and 5-HT7R in the hippocampus of rats with DHD;reduced the expression of 5-HT3R and TPH in the hippocampus,among which 5-HT3R expression was significantly reduced.This study suggests that PD can affect the function of hippocampus in rats with DHD by regulating the 5-HT signaling pathway.

关 键 词：白头翁汤 湿热泄泻 海马区 5-HT信号系统 

分 类 号：S852.2[农业科学—基础兽医学] S853.7[农业科学—兽医学]