白头翁散对泄泻仔猪结肠肠道菌群及肠道色氨酸代谢的影响  

Effects of Pulsatilla powder on intestinal flora and tryptophan metabolism in colon of piglets with diarrhea

作　　者：冯硕 张丽芳 谢雨霏 姜盛铭 曹立亭[1] 毕师诚 马琪 FENG Shuo;ZHANG Lifang;XIE Yufei;JIANG Shengming;CAO Liting;BI Shicheng;MA Qi(College of Veterinary Medicine,Southwest University,Rongchang,Chongqing 402460,China)

机构地区：[1]西南大学动物医学院,重庆荣昌402460

出　　处：《中国兽医学报》2025年第3期568-579,共12页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32202849)。

摘　　要：选用24只断奶仔猪随机分为空白组、模型组、自愈组、白头翁散(Pulsatilla powder,PP)组,以“外邪+内伤+疫毒”复合因素建立仔猪泄泻模型,并用PP治疗。造模期间,每日监测仔猪体质量、精神状态及粪便形态。试验结束后,采集结肠进行组织病理学观察及通过ELISA检测结肠组织炎症因子IL-1β、IL-6含量,16S rRNA分析结肠肠道菌群,RT-qPCR法测定结肠色氨酸代谢相关基因。结果显示,与模型组相比,PP能明显改善泄泻仔猪结肠病理状态,杯状细胞显著增多(P<0.05);显著降低泄泻仔猪结肠IL-1β、IL-6含量(P<0.05)。肠道菌群结果显示,PP升高了厚壁菌门(Firmicutes,P<0.01)、螺旋体门(Spirochaetota,P<0.01)和乳酸杆菌属(Lactobacillus,P<0.05)的丰度,降低了拟杆菌门(Bacteroidota,P<0.05)、普雷沃菌属(Prevotellaceae_NK3B31_group,P<0.05)和梭菌属(Clostridium_sensu_stricto_1,P<0.01)的丰度。同时,PP显著降低了泄泻仔猪结肠TPH1 mRNA表达水平(P<0.05),升高了AhR(P<0.05)、IL-22 mRNA表达水平。结果表明,PP可能通过调节结肠肠道菌群及肠道色氨酸代谢相关基因缓解仔猪泄泻,为后续探究仔猪泄泻病证防治提供数据支持。This study aims to investigate the effects of Pulsatilla powder(PP)on colonic intestinal flora and tryptophan metabolism in piglets with diarrhea.Twenty-four weaned piglets were randomly divided into the normal control group,model group,self-healing group,and PP group.The model of piglet diarrhea was established by the compound factor of external evil+internal injury+epidemic virus,and treated with PP.During the modeling period,the body weight,mental status and fecal patterns of piglets were monitored daily.After the experiment,the colon tissues were collected for histopathological observation,and the inflammatory factors IL-1βand IL-6 in the colon tissue was detected by ELISA,16S rRNA was used to analyze colonic intestinal flora and RT-qPCR was used to determine the expression of genes related to tryptophan metabolism in the colon.The results showed that,compared with the model group,PP significantly improved the pathological status of the colon in piglets with diarrhea,with elevated body mass and a significant increase in goblet cells(P<0.05);and significantly reduced the levels of IL-1βand IL-6 in the colon of diarrhea piglets(P<0.05).The results of intestinal flora showed that PP increased the abundance of Firmicutes(P<0.01),Spirochaeota(P<0.01)and Lactobacillus(P<0.05),reduced the abundance of Bacteroidota(P<0.05)、Prevotella_NK3B31_group(P<0.05)and Clostridium_sensu_stricto_1(P<0.01).Meanwhile,PP significantly reduced the expression level of TPH1mRNA(P<0.05),increased the expression levels of AhR(P<0.05)and IL-22 mRNA in the colon of diarrhea piglets.These results indicates that PP may alleviate diarrhea in piglets by regulating colonic intestinal flora and genes related to intestinal tryptophan metabolism.This study provides data support for the subsequent investigation of piglet diarrhea prevention and treatment.

关 键 词：泄泻 白头翁散 结肠组织 肠道菌群 色氨酸代谢 

分 类 号：S852.2[农业科学—基础兽医学] S859.7[农业科学—兽医学]