根皮苷改善鼠伤寒沙门菌诱导的小鼠盲肠炎症以及肠道屏障损伤  

Phlorizin improves cecal inflammation and intestinal barrier damage induced by Salmonella typhimurium in mice

作　　者：李童 孔丽娟 冯艳丽 成基 鞠天缘 韩佳雯 付守鹏 柳巨雄 胡桂秋 张昊龙[3] LI Tong;KONG Lijuan;FENG Yanli;CHENG Ji;JU Tianyuan;HAN Jiawen;FU Shoupeng;LIU Juxiong;HU Guiqiu;ZHANG Haolong(College of Veterinary Medicine,Jilin University,Changchun 130062,China;Animal Husbandry Station of Jiutai District,Changchun 130599,China;China-Japan Union Hospital,Jilin University,Changchun 130033,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]长春市九台区畜牧总站,吉林长春130599 [3]吉林大学中日联谊医院,吉林长春130033

出　　处：《中国兽医学报》2025年第3期587-593,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31702211)。

摘　　要：将40只成年雄性C57BL/6小鼠随机分为4组(10只/组),分别为对照组、鼠伤寒沙门菌(Salmonella typhimurium,S.typhimurium)组(ST组)、根皮苷(phlorizin,PHZ)+S.typhimurium组(PHZ+ST组)、PHZ组(80 mg/kg),通过形态学观察、HE染色、ELISA、免疫荧光和Western blot方法进行相关指标检测。结果显示,PHZ能够显著增加S.typhimurium诱导的小鼠盲肠指数,而降低小鼠的脾脏指数(P<0.05),并减少小鼠盲肠病理损伤。同时,PHZ也能够显著减少S.typhimurium在盲肠、脾脏、肠系膜淋巴结和肝脏中的定植(P<0.05)。通过ELISA检测S.typhimurium诱导的小鼠盲肠炎性因子的变化,结果显示PHZ也能够显著抑制由S.typhimurium诱导的IL-1β、INF-γ、TNF-α以及IL-6表达的升高(P<0.05)。免疫荧光和Western blot结果显示,PHZ能够显著增加S.typhimurium诱导的小鼠盲肠屏障Occludin、Claudin-3和ZO-1蛋白表达水平(P<0.05)。结果表明,根皮苷能够改善S.typhimurium感染引起的小鼠盲肠炎症以及肠道屏障受损。Forty adult male C57BL/6 mice were randomly divided into four groups:the control group,Salmonella typhimurium(S.typhimurium)(ST)group,phlorizin(PHZ)+S.typhimurium(ST)group,and PHZ(80 mg/kg)group,with 10 mice in each group.Morphological observation,HE staining,ELISA,immunofluorescence and Western blot were performed,the results showed that PHZ significantly increased the cecal index,decreased the spleen index of S.typhimurium-induced mice(P<0.05),and reduce the pathological damage of cecum in mice.Meanwhile,PHZ treatment also significantly reduced colonization of S.typhimurium in the cecum,spleen,mesenteric lymph nodes and liver(P<0.05).The results of ELISA showed that PHZ treatment also significantly inhibited the S.typhimurium-induced increase in the expression of IL-1β,INF-γ,TNF-αand IL-6 in the cecum of mice(P<0.05).Immunofluorescence and Western blot results showed that PHZ significantly increased the protein expression levels of Occludin,Claudin-3,and ZO-1 in the cecal barrier of mice induced by S.typhimurium(P<0.05).These results confirmed that phlorizin could improve cecal inflammation and intestinal barrier damage induced by S.typhimurium in mice.

关 键 词：根皮苷 S.typhimurium 炎性因子 肠道屏障 

分 类 号：S852.2[农业科学—基础兽医学] S852.61[农业科学—兽医学]