河北某种鸡场肿瘤病的病原检测及分析  

Detection and analysis of pathogens associated with neoplastic diseases in breeders in Hebei Province

作　　者：伊雪燕 周润雨 殷鹤予 顾宇华 匡华丽 李蕴玉[1] 贾青辉[1] 李佩国[1] YI Xueyan;ZHOU Runyu;YIN Heyu;GU Yuhua;KUANG Huali;LI Yunyu;JIA Qinghui;LI Peiguo(Hebei Province Key Laboratory of Preventive Veterinary Medicine,College of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066000,China)

机构地区：[1]河北科技师范学院动物科技学院河北省预防兽医学重点实验室,河北秦皇岛066000

出　　处：《中国兽医学报》2025年第3期594-601,共8页Chinese Journal of Veterinary Science

基　　金：河北省现代农业产业体系蛋禽创新团队资助项目(HBCT2024260209);河北省现代农业产业体系肉禽创新团队资助项目(HBCT2024270207)。

摘　　要：为了确定种鸡肿瘤病致死的病原,采集病死鸡的肝脏、脾脏等组织,运用PCR进行病原检测、测序及遗传进化分析。结果显示:3个样品网状内皮组织增殖病病毒(reticuloendotheliosis virus,REV)均为阴性,1、2号样品为禽白血病病毒(avian leukosis virus J,ALV-J)阳性,3号样品为ALV-J和马立克病病毒(Marek's disease virus,MDV)阳性,初步确定种鸡死于ALV-J感染或ALV-J和MDV混合感染。其中,ALV-J gp85基因与J亚群参考毒株核苷酸同源性最高,为87.70%~99.30%,并与J亚群参考毒株处于同一分支;氨基酸同源性为78.00%~96.20%,存在一定突变。MDV meq基因与特超强毒株(vv+)的核苷酸同源性最高,为98.00%~99.00%,与特超强毒株型(vv+)参考毒株处于同一分支;氨基酸同源性为95.90%~98.20%,且有多个突变位点,其中第176位氨基酸由Pro突变为Arg或Ala,破坏了病毒MDV meq原有的Pro重复序列,从而可能导致其毒力增强。本试验为该地区种鸡肿瘤性疫病的防控提供了参考。In order to determine the causative agents responsible for the lethality of tumor disease in breeders,liver,spleen and other tissues of dead chickens were collected,and pathogen detection,sequencing and genetic evolution analysis were carried out by PCR.The results showed that all three samples were negative for reticuloendotheliosis virus(REV),samples 1 and 2 wereavian leukosis virus subgroup J(ALV-J)positive,and sample 3 was positive for ALV-J and Marek's disease virus(MDV),which preliminarily determined that the breeder died of ALV-J infection or ALV-J and MDV mixed infection.Among them,the ALV-J gp85 gene had the highest nucleotide homology with the reference strain of ALV-J,ranging from 87.70%to 99.30%,and was in the same branch with the reference strain of subgroup J.Amino acid homology ranged from 78.00%to 96.20%,with some mutations.The nucleotide homology between the MDV meq gene and the vv+strain was the highest,which was 98.00%-99.00%,and they were in the same branch.The homology of amino acids was 95.90%-98.20%,and there were multiple mutation sites,among which the 176th amino acid destroyed the original Pro repeat of the virus MDV meq due to the mutation of Pro to Arg or Ala,which may lead to the enhancement of its virulence.This study will provide a reference for the prevention and control of neoplastic diseases in breeders in this region.

关 键 词：种鸡 肿瘤病 病原分析 ALV-J gp85基因 MDV meq基因 

分 类 号：S857.4[农业科学—临床兽医学]