粗糠柴果实的化学成分研究  

Chemical constituents from fruit of Mallotus philippensis

作　　者：郑文琳 李文依 陈烨琪 郭亚萍 贺震旦 顾继洪 吴炎 ZHENG Wenlin;LI Wenyi;CHEN Yeqi;GUO Yaping;HE Zhendan;GU Jihong;WU Yan(School of Pharmacy,Medical School,Shenzhen University,Shenzhen 518060,China;College of Pharmacy,Shenzhen Technology University,Shenzhen 518118,China;Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]深圳大学医学部药学院,广东深圳518060 [2]深圳技术大学药学院,广东深圳518118 [3]广州中医药大学科技创新中心,广东广州510405

出　　处：《中草药》2025年第6期1888-1895,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(82373751);深圳市博士后留深资助项目。

摘　　要：目的研究粗糠柴Mallotus philippensis果实的化学成分。方法采用硅胶、ODS、凝胶及半制备高效液相等色谱分离技术进行分离纯化,通过质谱、核磁共振波谱及圆二色谱等方法对化合物进行结构鉴定,采用肉汤微量稀释法评价化合物的抗菌活性。结果从粗糠柴果实的甲醇提取物中分离得到6个化合物,分别鉴定为粗糠柴黄酮P(1)、粗糠柴黄酮Q(2)、5-羟基-7-甲氧基二氢黄酮(3)、(2S)-7-hydroxy-5-methoxy-8-prenylflavanone(4)、(2R,3S,4R)-2,3,4-trimethyl-3-[(3E,7E,11E)-3,8,12,16-tetramethyl-3,7,11,15-heptadecatetraen-1-yl]cyclohexanone(5)和豆甾-4-烯-3-酮(6)。化合物1对新型隐球菌的最低抑菌浓度(minimum inhibitory concentration,MIC)值为128μg/mL。结论化合物1和2为新化合物,化合物5为新天然产物,化合物3和4为首次从大戟科植物中分离得到,化合物6为首次从野桐属植物中分离得到。化合物1对新型隐球菌有一定的抑制作用。Objective To investigate the chemical constituents of the fruit of Mallotus philippensis.Methods The compounds were separated and purified by a variety of chromatographic methods including silica gel ODS,gel and semi-preparative high performance liquid chromatography.Their structures were established based on mass spectrometry,nuclear magnetic resonance spectroscopy and circular dichroism.The antimicrobial activities of these isolates were evaluated by broth microdilution method.Results A total of six compounds were obtained from the methanol extract of the fruit of M.philippensis and identified as mallophilol P(1),mallophilol Q(2),5-hydroxy-7-methoxyflavanone(3),(2S)-7-hydroxy-5-methoxy-8-prenylflavanone(4),(2R,3S,4R)-2,3,4-trimethyl-3-[(3E,7E,11E)-3,8,12,16-tetramethyl-3,7,11,15-heptadecatetraen-1-yl]cyclohexanone(5)and stigmast-4-en-3-one(6),respectively.The minimum inhibitory concentration(MIC)value of compound 1 against Cryptococcus neoformans was 128μg/mL.Conclusion Compounds 1 and 2 are new compounds,compound 5 is a new natural product,compounds 3 and 4 are reported from the Euphorbiaceae plants for the first time,and compound 6 is reported from the genus Mallotus for the first time.Compound 1 shows certain antimicrobial effect against C.neoformans.

关 键 词：粗糠柴 大戟科 野桐属 抗菌活性 粗糠柴黄酮P 粗糠柴黄酮Q 5-羟基-7-甲氧基二氢黄酮 豆甾-4-烯-3-酮 

分 类 号：R284.1[医药卫生—中药学]