湖北金粟兰中的倍半萜类成分  

Sesquiterpenoids from Chloranthus henryi var.hupehensis

作　　者：刘帅[1] 苏栢麟 王晓霞 庄鹏宇 柳航[3] LIU Shuai;SU Bailin;WANG Xiaoxia;ZHUANG Pengyu;LIU Hang(Department of Pharmacy,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;School of Pharmacy,North China University of Science and Technology,Tangshan 063210,China;Department of Pharmacy,Nanjing Drum Tower Hospital,the Affiliated Hospital of Nanjing University Medical School,Nanjing 210008,China)

机构地区：[1]首都医科大学附属北京中医医院药学部,北京100010 [2]华北理工大学药学院,河北唐山063210 [3]南京大学医学院附属鼓楼医院药学部,江苏南京210008

出　　处：《中草药》2025年第6期1896-1902,共7页Chinese Traditional and Herbal Drugs

基　　金：河北省自然科学基金资助项目(H2022209046)。

摘　　要：目的研究湖北金粟兰Chloranthus henryi var.hupehensis的化学成分及其抗炎活性。方法采用硅胶、凝胶、HPLC等色谱技术进行提取分离与纯化,运用MS、NMR、ECD等波谱方法并结合文献数据,对化合物结构进行鉴定,采用脂多糖(lipopolysaccharides,LPS)诱导的鼠巨噬细胞RAW 264.7体外炎症模型进行抗炎活性评价。结果从该植物根部分离得到11个倍半萜类化合物,分别鉴定为4β,5α,9α-trihydroxy-7-oxoguaia-8(11)-en-12,9-olid(1)、8S,9S-环氧化白术内酯I(I 2)、6β,10α-dihydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide(3)、长尾粗叶木内酯A(4)、6α-hydroxyeudesma-4(15),7(11),8(9)-triene-12,8-olide(5)、白术内酯III(6)、chlorantene C(7)、chlomultin B(8)、dihydrocurcolone(9)、commiphorene B(10)、5β(H)-10α-methyl-8-oxoeudesma-4(15),7(11)-dien-12,6-olide(11)。结论化合物1为新的愈创木烷型倍半萜,命名为湖北金粟兰内酯N,化合物2~3、9~11为首次从金粟兰属植物中分离得到,化合物1~3、6~11为首次从湖北金粟兰中分离得到。化合物2对LPS诱导的鼠巨噬细胞RAW 264.7的一氧化氮(NO)产生有一定抑制作用,半数抑制浓度(median inhibition concentration,IC50)为(29.0±11.3)μmol/L。Objective To study the chemical constituents and anti-inflammatory activities of Chloranthus henryi var.hupehensis.Methods The compounds were extracted,isolated,and purified by column chromatography of silica gel,gel and HPLC,and their structures were identified using spectroscopic methods such as MS,NMR,and ECD combined with literature data.The in vitro antiinflammatory activities of all compounds were evaluated using the lipopolysaccharide(LPS)induced inflammatory model of RAW 264.7 mouse macrophage cells.Results A total of 11 sesquiterpenoids were isolated from the roots of C.henryi var.hupehensis,which were identified as 4β,5α,9α-trihydroxy-7-oxoguaia-8(11)-en-12,9-olid(1),8S,9S-epoxylatractylenolide II(2),6β,10α-dihydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide(3),lasianthuslactone A(4),6α-hydroxyeudesma-4(15),7(11),8(9)-triene-12,8-olide(5),atractylenolideⅢ(6),chlorantene C(7),chlomultin B(8),dihydrocurcolone(9),commiphorene B(10),5β(H)-10α-methyl-8-oxoeudesma-4(15),7(11)-dien-12,6-olide(11).Conclusion Compound 1 was a new guaiane-type sesquiterpene named chlorahupelactone N,while compounds 2—3,9—11 were isolated from Chloranthus genus for the first time,and compounds 1—3,6—11 were isolated from C.henryi var.hupehensis for the first time.Compound 2 inhibited the production of nitric oxide(NO)in LPS-induced RAW 264.7 mouse macrophage cells with a median inhibition concentration(IC50)value of(29.0±11.3)μmol/L.

关 键 词：湖北金粟兰 倍半萜类化合物 愈创木烷型倍半萜 抗炎 湖北金粟兰内酯N 8S 9S-环氧化白术内酯Ⅱ 

分 类 号：R284.1[医药卫生—中药学]