基于指纹图谱和多指标成分定量测定及化学计量学的温胆汤共煎与单煎混合汤剂对比研究  

Comparative study on co-decoction and single-decoction mixed decoction of Wendan Decoction based on fingerprint,multi-index component quantitative determination and chemometrics

作　　者：薛潭 许曼文 范雪花 孙嘉宁 施钧瀚[1] 王艳丽[1] 姚静[1,3,4,5] 刘瑞新 XUE Tan;XU Manwen;FAN Xuehua;SUN Jianing;SHI Junhan;WANG Yanli;YAO Jing;LIURuixin(Department of Pharmacy,First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450003,China;College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Province Engineering Research Center for Clinical Application,Evaluation and Transformation of Traditional Chinese Medicine,Zhengzhou 450000,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan&Education Ministry of P.R.China,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Key Laboratory of Traditional Chinese Medicine Clinical Pharmacy,Zhengzhou 450000,China;Engineering Research Center for Chinese Medicine Pharmaceutical and New Drug Development,Ministry of Education,Beijing 100029,China)

机构地区：[1]河南中医药大学第一附属医院药学部,河南郑州450003 [2]河南中医药大学药学院,河南郑州450046 [3]河南省中药临床应用、评价与转化工程研究中心,河南郑州450000 [4]河南中医药大学呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046 [5]河南省中药临床药学中医药重点实验室,河南郑州450000 [6]中药制药与新药开发教育部工程研究中心,北京100029

出　　处：《中草药》2025年第6期1956-1966,共11页Chinese Traditional and Herbal Drugs

基　　金：河南省自然科学基金项目杰出青年科学基金项目(242300421023);河南省卫生健康中青年学科带头人专项(HNSWJW-2020014);河南省卫生健康委国家中医临床研究基地科研专项(2021JDZY036);2022年青年岐黄学者培养项目(No.[2022]256);河南省卫生健康委国家中医临床研究基地科研专项(2021JDZY014)。

摘　　要：目的基于温胆汤指纹图谱和指标成分定量测定,结合化学计量学方法分析其共煎传统汤剂(traditional decoction,TD)与单煎混合TD化学成分的差异,为温胆汤等方药科学制备和质量控制提供参考。方法采用HPLC法建立温胆汤10批共煎TD与10批单煎混合TD的指纹图谱,并评价其相似度;测定温胆汤中腺苷、辛弗林、甘草苷、柚皮苷、橙皮苷、6-姜酚和环磷酸腺苷7种指标成分的含量;采用主成分分析(principal component analysis,PCA)、多维标度分析(multidimensional scaling analysis,MDSA)和正交偏最小二乘法-判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)对指标成分进行数据分析。结果以10批温胆汤共煎TD的指纹图谱生成共有模式图谱,对照共有模式图谱,共煎TD与单煎混合TD的指纹图谱相似度分别为相似度1:0.897±0.043、0.845±0.069(n=10,P<0.01),相似度2:0.842±0.083、0.759±0.274(n=10,P>0.05);通过指纹图谱及指标成分定量测定发现,温胆汤共煎TD与单煎混合TD在化学成分种类上未见明显差异,含量上温胆汤共煎TD中腺苷、辛弗林、甘草苷、橙皮苷、6-姜酚的含量高于单煎混合TD(P<0.01),分别为单煎混合TD的2.037、1.926、2.505、4.265、1.964倍,柚皮苷、环磷酸腺苷含量与单煎混合TD无显著性差异(P>0.05);PCA、MDSA和OPLS-DA结果一致,可将温胆汤共煎TD与单煎混合TD分为2类。结论所建立的HPLC指纹图谱结合PCA、MDSA和OPLS-DA及多成分定量测定能客观、有效地显示温胆汤共煎TD与单煎混合TD中主要成分的差异,可为温胆汤质量控制和临床合理用药提供参考。Objective Based on the fingerprint and quantitative determination of index components of Wendan Decoction,combined with chemometrics methods,the differences of chemical components between co-decoction traditional decoction(TD)and single decoction mixed TD were analyzed,which provided a reference for the scientific preparation and quality control of Wendan Decoction and other prescriptions.Methods The fingerprints of 10 batches of co-decocted TD and 10 batches of single-decocted mixed TD of Wendan Decoction were established by HPLC,and their similarities were evaluated.The contents of adenosine,synephrine,liquiritin,naringin,hesperidin,6-gingerol and adenosine cyclophosphate in Wendan Decoction were determined.Principal component analysis(PCA),multidimensional scaling analysis(MDSA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to analyze the data of the index components.Results The fingerprint of 10 batches of Wendan Decoction co-decoction TD was used to generate a common pattern map.Compared with common pattern map,the similarity of fingerprint of co-decoction TD and single decoction mixed TD was similarity 1:0.897±0.043 and 0.845±0.069(n=10,P<0.01);similarity 2:0.842±0.083,0.759±0.274(n=10,P>0.05).Through the fingerprint and quantitative determination of index components,it was found that there was no significant difference in the types of chemical components between the co-decoction TD and the single-decoction mixed TD.The contents of adenosine,synephrine,liquiritin,hesperidin and 6-gingerol in the co-decoction TD were higher than those in the singledecoction mixed TD(P<0.01).They were 2.037,1.926,2.505,4.265 and 1.964 times of single decoction mixed TD,respectively.There was no significant difference in the content of naringin and adenosine cyclophosphate between co-decoction TD and single decoction mixed TD(P>0.05).The results of PCA,MDSA and OPLS-DA were consistent,and the Wendan Decoction co-decoction TD and single decoction mixed TD could be divided into two categories.Co

关 键 词：温胆汤 指纹图谱 单煎与共煎 化学计量学 腺苷 辛弗林 甘草苷 柚皮苷 橙皮苷 6-姜酚 环磷酸腺苷 

分 类 号：R283.6[医药卫生—中药学]