甘肃引种栽培不同品种金银花的质量评价与代谢组学分析  

Quality evaluation and metabolomics analysis of different varieties of Lonicerae Japonicae Flos cultivated in Gansu Province

作　　者：张素珍 张亚萍 魏玉杰 陈耀 宋国士 翟玉生 张芳[4] 杨志刚 ZHANG Suzhen;ZHANG Yaping;WEI Yujie;CHEN Yao;SONG Guoshi;ZHAI Yusheng;ZHANG Fang;YANG Zhigang(School of Pharmacy,Lanzhou University,Lanzhou 730030,China;Gansu Agricultural Engineering Technology Research Institute,Wuwei 733006,China;Tongwei Qingliang Yuan Limited Liability Company of Lonicerae Japonicae Flos Industry Poverty Alleviation and Development,Dingxi 743300,China;Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]兰州大学药学院,甘肃兰州730030 [2]甘肃省农业工程技术研究院,甘肃武威733006 [3]通渭县清凉沅金银花产业扶贫开发有限公司,甘肃定西743300 [4]山东中医药大学,山东济南250355

出　　处：《中草药》2025年第6期2135-2147,共13页Chinese Traditional and Herbal Drugs

基　　金：甘肃省科技计划项目(22CX8NA003);国家药监局中药材及饮片质量控制重点实验室项目(2024GSMPA-KL08)。

摘　　要：目的对甘肃引种栽培的不同品种金银花Lonicerae Japonicae Flos进行品质分析,为金银花品种选育和产业化开发提供新思路。方法采用ITS2条形码序列分析11个不同品种金银花的种间差异;采用HPLC建立金银花特征图谱,并对13个样品的指标成分进行含量测定;采用超高效液相色谱-四级杆飞行时间质谱(UPLC-QTOF-MS/MS)进行非靶向代谢组学分析。结果通过ITS2序列对比分析发现,金花3号、豫金2号、豫花1号和华金6号不同品种间核苷酸序列位点碱基发生较大突变,其中华金6号有24处碱基差异。根据HPLC特征图谱,指认了特征成分绿原酸、异绿原酸A和异绿原酸C。不同品种金银花绿原酸、异绿原酸A、异绿原酸C和木犀草苷的含量存在差异,其中北花1号、华金6号、封花1号、金花3号与豫金1号金银花的酚酸类成分及木犀草苷含量均显著高于《中国药典》2020年版的规定。不同品种金银花代谢组学分析共筛选出21个差异代谢物,包括异绿原酸、山柰酚-O-芸香糖苷等。结论北花1号、华金6号、封花1号、金花3号与豫金1号金银花作为重点品种,可结合生产实践进行推广示范研究。系统分析了不同品种金银花中主要有效成分的异同,为金银花品种鉴定、质量控制和生产利用提供了科学依据。Objective In order to offer new insights for the breeding and industrialization of Jinyinhua(Lonicerae Japonicae Flos,LJF),the comprehensive quality evaluation of different varieties of LJF cultivated in Gansu Province was carried out.Methods ITS2 barcode sequence was used to analyze the interspecific differences of 11 different varieties of LJF medicinal materials.The characteristic chromatogram of LJF was established by high performance liquid chromatography(HPLC),and the content of indicator components in 13 samples was determined.Un-targeted metabolomics analysis was performed using ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS/MS).Results Through ITS2 sequence alignment,it was found that there were significant mutations in nucleotide sequence sites among different varieties of Jinhua 3,Yujin 2,Yuhua 1,and Huajin 6,with 24 nucleotide differences observed in Huajin 6.Chlorogenic acid,isochlorogenic acid A,and isochlorogenic acid C were identified in the HPLC characteristic chromatograms.Different varieties of LJF showed significant differences in the content of chlorogenic acid,isochlorogenic acid A,isochlorogenic acid C,and luteoloside.The phenolic acid and luteolin content of Beihua 1,Huajin 6,Fenghua 1,Jinhua 3,and Yujin 1 were significantly higher than those specified in the 2020 edition of the Chinese Pharmacopoeia.Metabolomics analysis showed a total of 21 differential metabolites,including isochlorogenic acid,kaempferol-O-rutinoside.Conclusion Based on comprehensive analysis,Beihua 1,Huajin 6,Fenghua 1,Jinhua 3,and Yujin 1 could be selected as key varieties for the further production practice.This study systematically evaluated the similarities and differences of the main active components in different varieties of LJF,which provided scientific basis for variety identification,quality control and production and utilization of LJF in Gansu Province.

关 键 词：金银花 ITS2 特征图谱 绿原酸 异绿原酸A 异绿原酸C 木犀草苷 差异代谢物 

分 类 号：R286.12[医药卫生—中药学]