Laser speckle contrast imaging with principal component and entropy analysis: a novel approach for depth-independent blood flow assessment  

作　　者：Yu.Surkov P.Timoshina I.Serebryakova D.Stavtcev I.Kozlov G.Piavchenko I.Meglinski A.Konovalov D.Telyshev S.Kuznetcov E.Genina V.Tuchin 

机构地区：[1]Institution of Physics,Saratov State University,Saratov 410012,Russia [2]Scientific Medical Center,Saratov State University,Saratov 410012,Russia [3]Laboratory of Laser Molecular Imaging and Machine Learning,Tomsk State University,Tomsk 634050,Russia [4]Institute for Bionic Technologies and Engineering,I.M.Sechenov First Moscow State Medical University,Moscow 119991,Russia [5]Institute of Biomedical Systems,National Research University of Electronic Technology,Zelenograd,Moscow 124498,Russia [6]Department of Human Anatomy and Histology,Cytology and Embryology,Institute of Clinical Medicine N.V.Sklifosovsky,I.M.Sechenov First Moscow State Medical University,Moscow 119991,Russia [7]Aston Institute of Materials Research,School of Engineering and Applied Science,Aston University,Birmingham B47ET,UK [8]Burdenko Neurosurgery Institute,Moscow 125047,Russia

出　　处：《Frontiers of Optoelectronics》2025年第1期1-19,共19页光电子前沿(英文版)

摘　　要：Current study presents an advanced method for improving the visualization of subsurface blood vessels using laser speckle contrast imaging (LSCI), enhanced through principal component analysis (PCA) filtering. By combining LSCI and laser speckle entropy imaging with PCA filtering, the method effectively separates static and dynamic components of the speckle signal, significantly improving the accuracy of blood flow assessments, even in the presence of static scattering layers located above and below the vessel. Experiments conducted on optical phantoms, with the vessel depths ranging from 0.6 to 2 mm, and in vivo studies on a laboratory mouse ear demonstrate substantial improvements in image contrast and resolution. The method’s sensitivity to blood flow velocity within the physiologic range (0.98-19.66 mm/s) is significantly enhanced, while its sensitivity to vessel depth is minimized. These results highlight the method’s ability to assess blood flow velocity independently of vessel depth, overcoming a major limitation of conventional LSCI techniques. The proposed approach holds great potential for non-invasive biomedical imaging, offering improved diagnostic accuracy and contrast in vascular imaging. These findings may be particularly valuable for advancing the use of LSCI in clinical diagnostics and biomedical research, where high precision in blood flow monitoring is essential.

关 键 词：Laser speckle imaging Speckle contrast ENTROPY Principal component analysis Blood flow velocity Vascular imaging 

分 类 号：R318[医药卫生—生物医学工程]