小麦TaPM19-B1基因的克隆与表达分析  

Cloning and Expression Analysis of TaPM19-B1 Gene in Wheat(Triticum aestivum)

作　　者：黄丽丹 汪德州 王小燕[1] 唐益苗[2] HUANG Li-Dan;WANG De-Zhou;WANG Xiao-Yan;TANG Yi-Miao(College of Agriculture,Yangtze University,Jingzhou 434200,China;Institute of Hybrid Wheat,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China)

机构地区：[1]长江大学农学院,荆州434200 [2]北京市农林科学院杂交小麦所,北京100097

出　　处：《农业生物技术学报》2025年第4期709-720,共12页Journal of Agricultural Biotechnology

基　　金：北京市农林科学院基因组学育种协同创新(KJCX201907-2);北京市农林科学院改革与发展课题(XMZZQN202401)。

摘　　要：近年来,通过对控制小麦(Triticum aestivum)籽粒大小和休眠的遗传基础研究发现了许多与其性状相关的基因。本研究以前期鉴定到的脱落酸(abscisic acid,ABA)诱导的小麦质膜19(ABA-induced wheat plasma membrane 19,AWPM19)基因家族成员TaPM19-B1(TraesCS5B02G566400)为基础,利用'中国春'的cDNA为模板克隆出全长为543 bp的TaPM19-B1基因,编码180个氨基酸。小麦原生质体亚细胞定位预测表明,TaPM19-B1蛋白定位于细胞膜。对TaPM19-B1启动子区域的顺式作用元件进行分析,结果显示,该区域存在多个与种子发育、逆境响应以及植物激素响应相关的调控元件。结合小麦RNA-Seq转录组数据和qPCR验证显示,TaPM19-B1基因在小麦根、茎、叶和穗中基本不表达,但在小麦籽粒发育后期显著表达,尤其在10和25 DAA(开花后天数,days after anthesis)籽粒中的表达量最高。此外,该基因在干旱、盐、热和ABA胁迫下表达量均上调。对2个大粒品种(千粒重超过50 g)和2个小粒品种(千粒重小于21 g)籽粒发育不同阶段的TaPM19-B1基因表达进行分析,结果发现,大粒品种中TaPM19-B1的表达量显著高于小粒品种(P<0.05)。本研究为进一步揭示TaPM19-B1基因在小麦生长发育及逆境胁迫响应中的分子调控机制提供了理论依据。In recent years,numerous genes associated with wheat(Triticum aestivum)grain size and dormancy have been identified through studies on the genetic basis controlling these traits.Based on abscisic acid(ABA)induced wheat plasma membrane 19(AWPM19)protein family member TaPM19-B1(TraesCS5B02G566400),this study cloned the full-length TaPM19-B1 gene(543 bp)from the cDNA of the'Chinese Spring'wheat variety,which encoded a protein of 180 amino acids.Subcellular localization prediction of wheat protoplasts indicated that the Ta PM19-B1 protein was localized to the cell membrane.Analysis of the cis-acting elements in the TaPM19-B1 promoter region revealed that the presence of multiple regulatory elements associated with seed development,stress response,and phytohormone signaling.RNASeq transcriptome data of wheat and qPCR validation demonstrated that TaPM19-B1 was scarcely expressed in the wheat root,stem,leaf,and spike,but was significantly expressed during the later stages of grain development,with the highest expression levels observed at 10 and 25 days after anthesis(DAA).Furthermore,the expression of TaPM19-B1 was significantly upregulated under drought,salt,heat,and ABA stress conditions.Additionally,an analysis of TaPM19-B1 gene expression at different stages of grain development in 2 large-grain varieties(1000-grain weight>50 g)and 2 small-grain varieties(1000-grain weight<21 g)revealed that TaPM19-B1 expression was significantly higher in the large-grain varieties(P<0.05).This study provides theoretical insights into the molecular regulatory mechanisms of TaPM19-B1 in wheat growth,development,and response to environmental stresses.

关 键 词：小麦 非生物胁迫 TaPM19-B1 种子大小 

分 类 号：S3[农业科学—农艺学] S512.1