梅花GATA基因家族鉴定及PmGATA12在耐寒性调控中的作用分析  

Identification of the Prunus mume GATA Gene Family and Analysis of the Role of PmGATA12 in Cold Tolerance Regulation

作　　者：刘钊良 张军 金小军 金松恒 董彬[4] LIU Zhao-Liang;ZHANG Jun;JIN Xiao-Jun;JIN Song-Heng;DONG Bin(Beijing Orient Landscape and Environment Co.,Ltd,Beijing 100020,China;Tongchuang Engineering Design Co.Ltd,Shaoxing 312000,China;Jiyang college,Zhejiang A&F University,Zhuji 311800,China;School of Landscape Architecture,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]北京东方园林环境股份有限公司,北京100020 [2]同创工程设计有限公司,绍兴312000 [3]浙江农林大学暨阳学院,诸暨311800 [4]浙江农林大学风景园林与建筑学院,杭州311300

出　　处：《农业生物技术学报》2025年第4期771-781,共11页Journal of Agricultural Biotechnology

基　　金：浙江农林大学学校科研发展基金(2017FR016)。

摘　　要：梅花(Prunus mume)是我国的传统名花,具有重要的观赏价值。GATA转录因子在植物生长发育以及非生物胁迫调控过程中发挥重要的作用。本研究对梅花GATA基因家族进行全基因组鉴定,并对低温处理后PmGATA12的表达模式及功能进行了解析。研究结果表明,通过全基因组分析鉴定出20个PmGATA基因,分布在7条染色体上;系统进化分析发现,PmGATA蛋白可分为3个亚家族,同一亚族成员具有相似的保守基序和基因结构。结合转录组和qRT-PCR表达分析,获得了4个差异表达的PmGATA基因(PmGATA6,PmGATA11,PmGATA12和PmGATA14)。选择差异表达的PmGATA12基因异源转化拟南芥(Arabidopsis thaliana),结果显示,过表达PmGATA12显著增强了转基因株系的耐寒性。本研究为梅花的耐寒育种提供了重要的基因资源,也为深入理解梅花耐寒性的分子机制提供了新的线索。Prunus mume,a traditional famous flower in China,holds significant ornamental value.GATA transcription factors play a crucial role in the regulation of plant growth and development as well as in the response to abiotic stress.In this study,a genome-wide identification of the GATA gene family in P.mume was conducted,and their expression patterns following cold treatment were systematically analyzed,with the functional characterization of PmGATA12 being comprehensively investigated.The results indicated that a total of 20 PmGATA genes were identified through whole-genome analysis,distribute across 7 chromosomes.Phylogenetic analysis revealed that PmGATA proteins could be divided into 3 subfamilies.Members of the same subfamily have similar conserved motifs and gene structures.Furthermore,by integrating transcriptome and qRT-PCR expression analysis,4 differentially expressed PmGATA genes(PmGATA6,PmGATA11,PmGATA12,and PmGATA14) were identified.The genetic transformation of Arabidopsis thaliana with PmGATA12 significantly enhanced the cold tolerance of the transgenic lines.This study provides valuable gene resources for the cold-resistance breeding of P.mume and offers new insights into the molecular mechanisms underlying its cold tolerance.

关 键 词：梅花 GATA 表达模式 耐寒 

分 类 号：S685.17[农业科学—观赏园艺]