骨质疏松对骨髓间充质干细胞增殖、迁移与分化能力的影响  

Effect of osteoporosis on proliferation,migration and differentiation of bone marrow mesenchymal stem cells

作　　者：石玉莲 刘文清 樊庭宇 刘家瑄 欧阳钧 戴景兴 Shi Yulian;Liu Wenqing;Fan Tingyu;Liu Jiaxuan;Ouyang Jun;Dai Jingxing(Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics&Guangdong Engineering Research Center for Translation of Medical 3D Printing Application&National Virtual&Reality Experimental Education Center for Medical Morphology(Southern Medical University)&National Key Discipline of Human Anatomy,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学基础医学院人体解剖学教研室暨广东省医学生物力学重点实验室/广东省医学3D打印应用转化工程技术研究中心/国家级医学形态学虚拟仿真实验教学中心(南方医科大学),广州510515

出　　处：《中国临床解剖学杂志》2025年第2期190-194,共5页Chinese Journal of Clinical Anatomy

基　　金：国家重点研发计划(2022YFF1202600)。

摘　　要：目的研究骨质疏松对小鼠骨髓间充质干细胞(mBMSCs)增殖、分化的影响,为骨质疏松的治疗提供理论和实验支持。方法30只C57BL/6小鼠随机分为假手术组和手术组,假手术组只去除卵巢周围脂肪,手术组去除卵巢。手术后8周分别取小鼠股骨和胫骨,分离培养mBMSCs,观察其增殖、分化的变化。结果骨质疏松小鼠的mBMSCs的增殖、迁移能力下降。碱性磷酸酶染色结果显示骨质疏松小鼠的mBMSCs成骨分化能力下降。结论骨质疏松模型小鼠mBMSCs的增殖、迁移和分化能力下降。Objective To study the effect of osteoporosis on the proliferation and differentiation of mouse bone marrow mesenchymal stem cells(mBMSCs),and to provide theoretical and experimental support for the treatment of osteoporosis.Methods Thirty C57BL/6 mice were randomly divided into sham operation group and operation group.Sham operation group only removed the fat around the ovary,and operation group removed the ovary.mBMSCs were taken from the femur and tibia of mice and cultured 8 weeks after operation to observe the changes of proliferation and differentiation.Results The proliferation and migration ability of mBMSCs in osteoporosis mice were decreased.The results of alkaline phosphatase staining showed that mBMSCs had decreased osteogenic differentiation ability in osteoporosis mice.Conclusions The proliferation,migration,and differentiation ability of mBMSCs in osteoporotic mice were decreased.

关 键 词：骨质疏松 小鼠骨髓间充质干细胞(mBMSCs) 增殖 迁移 分化 

分 类 号：R681[医药卫生—骨科学]