电针干预缓解高脂血症模型大鼠肝脏脂质积累和肠黏膜损伤  

Electroacupuncture Alleviates Hepatic Lipid Accumulation and Intestinal Mucosal Injury in Hyperlipidemia Model Rats

作　　者：武欢 张照庆 居诗如 王小飞 刘志刚 周丽 张红星 林威 Wu Huan;Zhang Zhaoqing;Ju Shiru(School of Traditional Chinese Medicine,Faculty of Medicine/Institute of Acupuncture and Moxibustion,Jianghan University,Wuhan 430056,China;Department of Pain and Rehabilitation,Wuhan Third Hospital,Wuhan 430074,China)

机构地区：[1]江汉大学医学部中医学院/江汉大学针灸研究所,武汉460056 [2]武汉市第三医院疼痛康复科,武汉430074 [3]湖北省第三人民医院儿童康复科,武汉430033

出　　处：《华中科技大学学报(医学版)》2025年第2期166-171,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.82274663);武汉市自然科学基金资助项目(No.2024040801020370);湖北省自然科学基金资助项目(No.2022CFB468)。

摘　　要：目的探讨电针对高脂血症模型大鼠肝脏和肠黏膜损伤的缓解作用。方法将Wistar大鼠分为对照组、模型组、电针组,每组6只。模型组和电针组均采用高脂饲料喂养,电针组在造模5周后进行电针干预8周。治疗结束后,苏木精-伊红(HE)染色检测各组大鼠肝脏和回肠黏膜组织病理变化;生化检测大鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量;AB-PAS染色检测大鼠回肠黏膜组织;Western blot检测大鼠回肠组织法尼醇X受体(FXR)、成纤维细胞生长因子19(FGF19)、ZO-1、Occludin和连接粘附分子C(JAM-C)的蛋白表达。结果与对照组比较,模型组大鼠体重显著增加(P<0.05);肝脏和回肠组织出现严重病变;血清中TC、TG和LDL-C水平升高,回肠组织中JAM-C蛋白表达增加(均P<0.01);血清中HDL-C水平降低,回肠组织中FXR、FGF19、ZO-1和Occludin蛋白表达下降(均P<0.01)。与模型组比较,电针组大鼠体重增加显著减少(P<0.01);肝脏和回肠组织病理损伤减轻;血清中TC、TG和LDL-C水平降低,回肠组织中JAM-C蛋白表达下降(均P<0.01);血清中HDL-C水平升高,回肠组织中FXR、FGF19、ZO-1和Occludin蛋白表达增加(均P<0.01)。结论电针刺激通过激活大鼠FXR/FGF19通路,从而改善肠道屏障损伤,发挥对肝脏的保护作用,减轻血脂异常。Objective To investigate the effect of electrotherapy on liver and intestinal mucosal injury in hyperlipidemia rats.Methods Wistar rats were divided into control group,model group and electroacupuncture group,with 6 rats in each group.Both the model group and the electroacupuncture group were fed with high-fat diet,and the electroacupuncture group received electroacupuncture intervention.After treatment,HE staining was used to detect the pathological changes of liver and ileal mucosa.The contents of TC,TG,LDL-C and HDL-C in serum of rats were detected by biochemical method.AB-PAS staining was used to detect ileum mucosa in rats.The protein expressions of FXR,FGF19,ZO-1,occludin and JAM-C in rat ileum were detected by Western blotting.Results Compared with the control group,the body weight was significantly increased in the model group(P<0.05),and the liver and ileum tissues were seriously damaged.The levels of TC,TG and LDL-C in serum and the expression of JAM-C protein in ileum were increased(P<0.01).HDL-C level in serum,FXR,FGF19,ZO-1 and occludin in ileum tissue were decreased(P<0.01).Compared with model group,the weight gain of rats in electroacupuncture group was significantly reduced(P<0.01),and the pathological injury of liver and ileum tissue was alleviated.The levels of TC,TG and LDL-C in serum and the expression of JAM-C protein in ileum were decreased(P<0.01).HDL-C level in serum as well as FXR,FGF19,ZO-1 and occludin levels in ileum tissue were increased(P<0.01).Conclusion Electroacupuncture stimulation can improve the damage of intestinal barrier by activating FXR/FGF19 pathway in rats,play a protective role in liver,and inhibit dyslipidemia.

关 键 词：高血脂症 电针 FXR/FGF19通路 肠道屏障 

分 类 号：R245[医药卫生—针灸推拿学]