D-半乳糖致心脏衰老动物模型的建立及评估  

Establishment and Evaluation of an Animal Model of Heart Aging Induced by D-galactose

作　　者：王柏惠 王经 王琦 黎静 谭机永[1] Wang Baihui;Wang Jing;Wang Qi(Key Laboratory of Longevity and Aging-related Diseases,Ministry of Education,Guangri Medical University,Nanning 530021,China;Department of Physiology,School of Basic Medical Sciences,Guangri Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学转化医学研究中心,长寿与老年相关疾病教育部重点实验室,南宁530021 [2]广西医科大学基础医学院生理学教研室,南宁530021

出　　处：《华中科技大学学报(医学版)》2025年第2期211-216,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广西壮族自治区自然科学基金资助项目(No.2024GXNSFAA010074)。

摘　　要：目的采用D-半乳糖(D-galactose,D-gal)制备心脏衰老动物模型,并对该模型进行评价。方法将30只小鼠随机分为两组,每组15只。其中实验组(D-gal组)采用颈背部皮下注射D-gal溶液(150 mg/kg,每日1次),建立心脏衰老小鼠模型。对照组(Vehicle组)采用颈背部注射等量0.9%氯化钠注射液。每周称重1次,按照体重调整给药剂量,并利用超声影像、病理组织学染色、Western blot等技术对模型表型进行评估。结果实验组小鼠一般情况、心腔大小、射血分数、衰老相关蛋白表达量均有明显变化,接近心脏自然衰老的各项指标,无小鼠死亡。结论利用D-半乳糖建立心脏衰老动物模型是可行的,该方法简便易行、成模时间较短、成功率较高,是建立心脏衰老动物模型的一种较好的方法。Objective To establish and evaluate an animal model of cardiac aging by D-galactose(D-Gal).Methods Thirty mice were randomly divided into two groups,with 15 mice in each group.The experimental group(D-gal group)was injected with D-gal solution(150 mg/kg,once a day)on the back of neck to establish the cardiac aging mice model.The control group(Vehicle group)was injected with 0.9%sodium chloride injection on the neck and back.Weight was measured once a week,and the dose was adjusted according to body weight,and the phenotype of the model was evaluated by ultrasound,histopathological staining,Western blotting and other techniques.Results In the experimental group,there were significant changes in general condition,cardiac cavity size,ejection fraction and aging-related protein expression,which were consistent with the indexes of natural aging of the heart.No mice death was observed.Conclusion It is feasible to establish heart aging animal model with D-galactose.This method has the advantages of simple operation,short modeling time and high success rate,which is a satisfying method to establish heart aging animal model.

关 键 词：D-半乳糖 心脏衰老 衰老小鼠模型 

分 类 号：R339.38[医药卫生—人体生理学]