湖北雪茄烟草根腐病的病原鉴定  

Identification of root rot pathogen in Hubei cigar tobacco

作　　者：黎妍妍 杨雯婷 许汝冰 覃耀 黄俊斌[2] 乔保明 吕培军 秦铁伟[5] 杨勇[6] LI Yanyan;YANG Wenting;XU Rubing;QIN Yao;HUANG Junbin;QIAO Baoming;LÜPeijun;QIN Tiewei;YANG Yong(Tobacco Research Institute of Hubei Province,Wuhan 430030,China;College of Plant Science and Technology,Hubei Provincial Key Laboratory for Crop Disease Monitoringand Safety Control,Huazhong Agriculture University,Wuhan 430207,China;Enshi Branch of Hubei Province Tobacco Company,Enshi 445000,Hubei,China;Shiyan Branch of Hubei Province Tobacco Company,Shiyan 442000,Hubei,China;Yichang Branch of Hubei Province Tobacco Company,Yichang 443000,Hubei,China;College of Life Sciences,State Key Laboratory of Biocatalysis and Enzyme Engineering,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北省烟草科学研究院,武汉市430030 [2]华中农业大学植物科技学院,湖北省作物病害监测和安全控制重点实验室,武汉市430207 [3]湖北省烟草公司恩施州公司,湖北省恩施市445000 [4]湖北省烟草公司十堰市公司,湖北省十堰市442000 [5]湖北省烟草公司宜昌市公司,湖北省宜昌市443000 [6]湖北大学生命科学学院,省部共建生物催化与酶工程国家重点实验室,武汉市430062

出　　处：《烟草科技》2025年第4期45-52,共8页Tobacco Science & Technology

基　　金：湖北省烟草公司重点科技项目“雪茄烟主要病虫害灾变规律调查及防控技术研究与应用”(027Y2022-023);中国烟草总公司重大科技项目“烟草镰刀菌根腐病成灾机制及绿色高效防控技术研发与应用”[110202401013(LS-03)]。

摘　　要：为明确引起湖北雪茄烟草根腐病的病原,在湖北省雪茄烟草主产区丹江口市、恩施州和宜昌市采集根腐病样品,采用常规组织分离法分离病原菌,通过室内带菌土壤接种验证其致病性,并进行了形态学和分子生物学鉴定。结果表明,镰刀菌在PDA培养基上菌落为白色至淡粉色,后期部分颜色加深,菌丝呈绒状。分生孢子呈典型的镰刀状,大型分生孢子多隔、无色、基本为镰刀形;小型分生孢子大部分无隔,呈椭圆形或纺锤形。基于ITS、EF-1α序列的系统发育分析,鉴定出引起湖北雪茄烟草根腐病的主要病原为尖孢镰刀菌(Fusariumoxysporum)和茄病镰刀菌(Fusariumsolani)。In order to clarify the pathogen causing root rot in Hubei cigar tobacco,diseased plant samples were collected from Danjiangkou,Enshi and Yichang regions,which are the main producing areas of Hubei cigar tobacco.The pathogen was isolated by conventional tissue isolation method,and its pathogenicity was verified by indoor inoculation of soil containing pathogen,and morphological identification and molecular biological identification were carried out.The results showed that the colonies of Fusarium on PDA medium were white to flesh-pink,and in some of them,the color deepened at a later stage and the mycelium was floccose.The conidia were typically sickle-shaped,the macroconidia were mostly septate,colorless,and sickle-shaped.Most of the microconidia were aseptate,elliptic or fusiform.Phylogenetic analysis based on ITS and EF-1αsequences identified Fusariumoxysporum and Fusariumsolani as the main pathogens causing root rot in Hubei cigar tobacco.

关 键 词：雪茄烟草 根腐病 致病性 形态学鉴定 分子生物学鉴定 系统发育 

分 类 号：S43[农业科学—农业昆虫与害虫防治]