水稻蔗糖转运蛋白OsSUT4基因的克隆与功能分析  

Cloning and Function Analysis of Rice Sucrose Transporter OsSUT4 Gene

作　　者：蔡昱萌 汪颖 何飞 刘燕清 刘静妍 郭彦丽 孙玥 苏京平[1,3] 王胜军 李军玲[1,3] CAI Yumeng;WANG Ying;HE Fei;LIU Yanqing;LIU Jingyan;GUO Yanli;SUN Yue;SU Jingping;WANG Shengjun;LI Junling(Crop Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China;Tianjin Agricultural University,Tianjin 300392,China;Tianjin Key Laboratory of Crop Genetics and Breeding,Tianjin 300384)

机构地区：[1]天津市农业科学院农作物研究所,天津300384 [2]天津农学院,天津300392 [3]天津市农作物遗传育种重点实验室,天津300384

出　　处：《河南农业科学》2025年第3期1-7,共7页Journal of Henan Agricultural Sciences

基　　金：天津市农业科学院青年科技创新项目(2021013,2021004,2021007)。

摘　　要：克隆水稻蔗糖转运蛋白OsSUT4基因,利用酵母异源表达系统验证OsSUT4蔗糖转运功能,并在水稻中过表达,观察转基因植株表型,鉴定其耐盐性和抗旱性,探究OsSUT4基因在水稻抗逆中的作用,为水稻遗传改良提供新的基因资源和理论依据。结果表明,OsSUT4基因主要在拔节期的剑叶中表达;利用酵母异源表达系统首次验证了OsSUT4具有蔗糖转运功能;过表达OsSUT4基因没有明显改变籽粒的形态和千粒质量,但显著提高了株高和盐、干旱胁迫条件下的存活率,增强了水稻的耐盐性和抗旱性。The rice sucrose transporter gene OsSUT4 was cloned,and sucrose transport capability of OsSUT4 was verified by yeast heterologous expression system.The gene was overexpressed in rice,the phenotype of transgenic plants was observed,and the salt tolerance and drought resistance was identified,so as to explore the role of the OsSUT4 gene in stress resistance in rice and provide new gene resources and theoretical foundations for rice genetic improvement.The results showed that OsSUT4 gene was primarily expressed in the flag leaves at jointing stage;The sucrose transport capability of OsSUT4 was confirmed for the first time using the yeast heterologous expression system;Overexpression of OsSUT4gene did not significantly alter the grain morphology or the thousand-grain weight,but significantly increased plant height and survival rates under salt and drought stresses,thereby enhancing the salt tolerance and drought resistance of rice.

关 键 词：水稻 蔗糖转运蛋白 过表达 株高 耐盐性 抗旱性 

分 类 号：S511[农业科学—作物学]