不同质量浓度外源6-BA对黄精初生根茎生理特性的影响  

Effects of 6-BA on Physiological Characteristics of Primary Rhizomes of Polygonatum sibiricum Red

作　　者：王溶溶 岳贺伟 张博 胡鹏 刘旸 杨雪 李娟[1] 李连珍[1,2] WANG Rongrong;YUE Hewei;ZHANG Bo;HU Peng;LIU Yang;YANG Xue;LI Juan;LI Lianzhen(College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China;Xinyang Academy of Agricultural Sciences,Xinyang 464000,China)

机构地区：[1]河南农业大学农学院,河南郑州450046 [2]信阳市农业科学院,河南信阳464000

出　　处：《河南农业科学》2025年第3期60-70,共11页Journal of Henan Agricultural Sciences

基　　金：河南省中药材产业科技特派员服务团项目(30803655/2024)。

摘　　要：为探究6-苄氨基嘌呤(6-benzylaminopurine,6-BA)对黄精(Polygonatum sibiricum Red.)初生根茎生理特性的影响,设置0 mg/L(CK)、50 mg/L(M1)、100 mg/L(M2)、200 mg/L(M3)4个质量浓度的6-BA处理黄精初生根茎,调查初生根茎出苗及生长情况,测定其出苗过程中过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、脂肪酶、苹果酸脱氢酶(MDH)、6-磷酸葡萄糖脱氢酶(G6PDH)活性及丙二醛(MDA)、脂肪、游离氨基酸、蛋白质、淀粉、可溶性糖含量。结果表明,6-BA处理的黄精初生根茎出苗率,幼苗的叶长、叶宽、初生根茎、直径、主根长、侧根数和干物质量均显著高于CK(P<0.05)。其中,第61天,M1、M2、M3、CK出苗率分别为33.10%、60.12%、46.66%、3.68%,M2处理与其他处理相比差异显著(P<0.05)。第1—31天,6-BA处理的黄精初生根茎可溶性糖、淀粉、蛋白质、游离氨基酸和脂肪含量均显著低于CK(P<0.05),脂肪酶活性显著高于CK(P<0.05);POD、SOD、CAT、MDH和G6PDH酶活性整体上高于CK,MDA含量整体上低于CK。SOD、CAT和G6PDH活性均在处理后第41天达到最高值。其中,M1、M2、M3处理SOD活性分别比CK提高39.87%、86.99%、51.02%;CAT活性分别比CK提高30.25%、54.06%、45.79%;G6PDH活性分别比CK提高51.90%、132.42%、76.89%。相关性和主成分分析结果表明,淀粉含量、可溶性糖含量、蛋白质含量、游离氨基酸含量、脂肪含量、过氧化物酶活性、超氧化物歧化酶活性和过氧化氢酶活性这8个指标在不同质量浓度6-BA(0、50、100、200 mg/L)处理下可作为表征黄精初生根茎出苗及生长的核心评价指标。综上,6-BA能够显著提高黄精初生根茎出苗率、物质代谢水平及抗氧化酶活性,其最佳质量浓度为100 mg/L。To investigate the effects of 6-benzylaminopurine(6-BA)on the physiological characteristics of the initial root tubers of Polygonatum sibiricum Red.,four mass concentrations of 6-BA(0 mg/L,CK;50mg/L,M1;100 mg/L,M2;200 mg/L,M3)were applied to the initial root tubers.The germination and growth of the initial root tubers were observed,and the activities of peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),lipase,malate dehydrogenase(MDH),6-phosphogluconate dehydrogenase(G6PDH),and the contents of malondialdehyde(MDA),lipids,free amino acids,proteins,starch,and soluble sugars were measured.The results showed that the germination rate,seedling leaf length,leaf width,diameter,main root length,lateral root number,and dry weight of the primary rhizomes treated with6-BA were significantly higher than those in the CK group(P<0.05).On the 61st day,the emergence rates of M1,M2,M3,and CK were 33.10%,60.12%,46.66%and 3.68%;Among them,the M2 treatment showed significant differences compared to other treatments(P<0.05).On days 1—31,the soluble sugar,starch,protein,free amino acids,and fat content in the primary rhizomes of Polygonatum sibiricum Red.treated with 6-BA were significantly lower than those in the CK group(P<0.05),and the lipase activity was significantly higher than that in the CK group(P<0.05);The activities of POD,SOD,CAT,MDH,and G6PDH enzymes in the primary rhizomes of Polygonatum sibiricum Red.treated with 6-BA were higher than those in the CK group,while the MDA content was lower.The activities of SOD,CAT,and G6PDH reached their highest values on the 41st day after treatment.Among them,the SOD activity in M1,M2,and M3 increased by 39.87%,86.99%,and 51.02%,respectively,compared to the CK group;The CAT activity in M1,M2,and M3 increased by 30.25%,54.06%,and 45.79%respectively compared to CK;The G6PDH activity in M1,M2,and M3 increased by 51.90%,132.42%,and 76.89%respectively compared to CK.The results of correlation and principal component analysis showed that the eight indicators of starch content,soluble

关 键 词：黄精 6-苄氨基嘌呤 初生根茎出苗 上胚轴休眠 生理特性 

分 类 号：S567.239[农业科学—中草药栽培]