植物根际促生菌和褪黑素对大花金鸡菊抗氧化特性和砷吸收的影响  

Effect of PGPR and Melatonin on the Antioxidant Properties and Arsenic Absorption of Coreopsis grandiflora

作　　者：梁亚男[1] 邬梦晞 肖雍琴[1] 曾鲸津 段益莉[1] LIANG Ya’nan;WU Mengxi;XIAO Yongqin;ZENG Jingjin;DUAN Yili(Neijiang Vocational and Technical College,Neijiang 641000,China;College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]内江职业技术学院,四川内江641000 [2]四川农业大学风景园林学院,四川成都611130

出　　处：《河南农业科学》2025年第3期87-98,共12页Journal of Henan Agricultural Sciences

基　　金：四川省教育厅2022-2024年职业教育人才培养和教育教学改革研究项目(GZJG2022-312)。

摘　　要：以大花金鸡菊为试材,采用室内盆栽试验,探究80 mg/kg砷(As)胁迫下接种植物根际促生菌(PGPR)与叶面喷施5μmol/L(MT_(1))、20μmol/L(MT_(2))、50μmol/L(MT_(3))褪黑素(MT)及其复合处理对大花金鸡菊生长、根系构型、叶绿素含量及叶绿素荧光参数、营养元素、As吸收及抗氧化特性的影响。结果表明,与无As胁迫的CK处理相比,As胁迫下大花金鸡菊株高、叶面积、总生物量(地上部和根系干质量)整体上显著降低。叶面喷施不同浓度MT和MT+PGPR处理可以减轻As胁迫对大花金鸡菊的伤害。As胁迫下,与不接种PGPR不喷施MT处理相比,As+MT_(2)+PGPR处理的大花金鸡菊根系构型(总根长、总体积、总表面积、根尖数)显著改善,根系活力提高了166.1%,叶绿素含量、PSⅡ最大光化学效率(Fv/Fm)、光化学淬灭系数(q P)分别增加56.7%、15.6%和62.8%,而非光化学淬灭系数(NPQ)下降了23.7%。As胁迫显著降低了大花金鸡菊地上部和根系中氮、磷、钾含量,As+MT_(2)+PGPR处理能不同程度地增加地上部和根系中氮、磷、钾含量。同时,叶面喷施不同浓度MT和MT+PGPR处理能够增加大花金鸡菊地上部和根系As含量,提高大花金鸡菊As转运系数和富集系数,激活超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性并增加抗坏血酸(As A)和谷胱甘肽(GSH)含量,从而缓解大花金鸡菊受到的氧化胁迫。综上,As+MT_(2)+PGPR处理提高大花金鸡菊抵抗As污染的效果最好。Using Chaoyang seedlings of Coreopsis grandiflora as test materials,an indoor pot experiment was conducted to investigate the effects of inoculating plant growth promoting bacteria(PGPR)and foliar spraying of melatonin(MT)at concentrations of 5μmol/L(MT_1),20μmol/L(MT_2),and 50μmol/L(MT_3)under 80 mg/kg arsenic(As)stress on the growth,root architecture,chlorophyll content,chlorophyll fluorescence parameters,nutrient elements,As absorption,and antioxidant properties of C.grandiflora.The results showed that compared with the CK treatment without As stress,the overall plant height,leaf area,and total biomass(aboveground and root dry weight)of C.grandiflora were significantly reduced under As stress.Foliar spraying of different concentrations of MT and MT+PGPR treatment could alleviate the damage of As stress to C.grandiflora.Under As stress,compared with the treatment without PGPR inoculation or MT spraying,the root system configuration(total root length,total volume,total surface area,root tip number)of C.grandiflora treated with As+MT_2+PGPR was significantly improved.The root activity increased by 166.1%,and the chlorophyll content,maximum photochemical efficiency(Fv/Fm)of PSⅡ,and photochemical quenching coefficient(q P)increased by 56.7%,15.6%,and 62.8%,respectively,while the non photochemical quenching coefficient(NPQ)decreased by 23.7%.As stress significantly reduced the nitrogen,phosphorus,and potassium content in the aboveground and root systems of C.grandiflora,while As+MT_2+PGPR treatment could increase the nitrogen,phosphorus,and potassium content in the aboveground and root systems to varying degrees.At the same time,foliar spraying of different concentrations of MT and MT+PGPR could increase the As content in the aboveground and root parts of C.grandiflora,improve the As transport coefficient and enrichment coefficient,activate the activities of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),and glutathione reductase(GR),and increase the content of ascorbic acid(As A)and glutathi

关 键 词：大花金鸡菊 植物根际促生菌 褪黑素 砷胁迫 根系构型 抗氧化酶 

分 类 号：S682[农业科学—观赏园艺]