牡丹PomiR2592bj及其靶基因PoSDR1的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of PomiR2592bj and Its Target Gene PoSDR1 of Tree Peony

作　　者：雷炀 高靖姗 南琼琼 郭丽丽[1] 侯小改[1] LEI Yang;GAO Jingshan;NAN Qiongqiong;GUO Lili;HOU Xiaogai(College of Agriculture,Henan University of Science and Technology,Luoyang 471023,China;Luoyang Agricultural Development Investment Group Co.,Ltd.,Luoyang 471023,China)

机构地区：[1]河南科技大学农学院,河南洛阳471023 [2]洛阳农发投资集团有限公司,河南洛阳471023

出　　处：《河南农业科学》2025年第3期120-128,共9页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(U1804233);河南省高校科技创新人才项目(22HASTIT036)。

摘　　要：基于前期对牡丹早花品种凤丹、凤丹早花突变植株和晚花品种连鹤转录组、miRNA和降解组联合分析,挖掘到调控牡丹花期的PomiR2592bj及其靶基因PoSDR1。为探究牡丹PomiR2592bj和PoSDR1的生物学特性及功能,克隆得到了113 bp的PomiR2592bj和1 380 bp的PoSDR1序列,二级结构分析表明,PomiR2592bj的前体序列可形成稳定的茎环结构,成熟序列分布在其3'臂端;多序列比对表明,PomiR2592bj成熟序列具有保守性;系统进化树分析显示,miR2592家族分为了10个分支,且具有同源性。PoSDR1基因编码的蛋白质含296个氨基酸,具有亲水性,不存在跨膜结构域,不存在信号肽,Ser位点有19个被磷酸化,Thr位点有11个被磷酸化,Tyr位点有2个被磷酸化,具有NADB_Rossmann超家族保守域。PomiR2592bj经预测与PoSDR1具有靶向关系。Based on the previous analysis of transciptome,miRNA and degradome of early-flowering Fengdan,early-flowering Fengdan mutant plants,and late-flowering Lianhe,we identified PomiR2592bj and its target gene PoSDR1,which were excavated to regulate the flowering period of tree peony.To further elucidate the biological characteristics and functions of PomiR2592bj and PoSDR1,we cloned a113 bp sequence for PomiR2592bj and a 1 380 bp sequence for PoSDR1.Secondary structure analysis revealed that the precursor sequence of PomiR2592bj could form a stable stem-loop structure with mature sequences located at the 3' arm end;multiple sequence alignment indicated conservation within these mature sequences;phylogenetic tree analysis unveiled that the miR2592 family was subdivided into 10branches with homology.The protein encoded by PoSDR1 contained 296 amino acids.The protein was hydrophilic with no transmembrane domain and signal peptide.19 Ser sites of PoSDR1 were phosphorylated,while 11 Thr sites were phosphorylated,and 2 Tyr sites were phosphorylated.PoSDR1had a NADB_Rossmann superfamily conserved domain.PomiR2592bj was predicted to have a targeting relationship with PoSDR1.

关 键 词：牡丹 PomiR2592bj PoSDR1 克隆 生物信息学 

分 类 号：S682.19[农业科学—观赏园艺]