隐性白羽和矮小基因分子标记辅助技术在新资源罗坑鸡的应用  

Application of Molecular Marker⁃assisted Technique of Recessive White Feather and Dwarf Gene in New Resource Luokeng Chicken

作　　者：严霞 王春兰 李静 许欣纯 陈伟森 何燕华 陈细浩 YAN Xia;WANG Chunlan;LI Jing;XU Xinchun;CHEN Weisen;HE Yanhua;CHEN Xihao(Institute of Animal Science,Guangdong Academy of Agricultural Sciences,State Key Laboratory of Livestock and Poultry Breeding,Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangzhou Guangdong 510640;Shaoguan Institute of Animal Husbandry Science,Shaoguan Guangdong 512000)

机构地区：[1]广东省农业科学院动物科学研究所/猪禽种业全国重点实验室/广东省畜禽育种与营养研究重点实验室,广东广州510000 [2]韶关市畜牧研究所,广东韶关512000

出　　处：《广东畜牧兽医科技》2025年第2期66-70,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省级乡村振兴战略专项(2023⁃XBH⁃06⁃002);韶关市科技计划项目(220610144531382);广东省农业科学院专家工作站(2023工作站11)。

摘　　要：为了快速摸清罗坑鸡新资源的品种特性,该文利用分子标记辅助技术对罗坑鸡群体进行隐性白羽基因和矮小基因PCR鉴定。在356只群体样本中,检测出含隐性白羽基因杂合型的个体46只,F0、F1世代含隐性白羽基因杂合子频率分别为11%、13.67%,未检测出含矮小基因的个体。利用分子标记辅助技术可快速淘汰杂合子,有效缩短地方种鸡提纯复壮周期,为罗坑鸡种质资源保护评鉴、开发利用奠定理论基础。To quickly find out the variety characteristics of the new resources of Luokeng chicken,in this study,molecular assisted marker technology involving recessive white⁃feathered(TYR)allele and Z⁃linked dw allele were performed.Consequently,46 heterozygous recessive white⁃feathered individuals were detected,the frequency of the heterozygous recessive white⁃feathered allele in the F0 and F1 generation were 11%and 13.67%,respectively and no Z⁃linked dw allele individual were detected across 356 samples.Altogether,the potential recessive white⁃feathered heterozygotes were totally eliminated within one generation,thus laying a foundation for the further genetic protection of Luokeng chicken.

关 键 词：TYR基因 DW基因 分子辅助标记 罗坑鸡 

分 类 号：S813.3[农业科学—畜牧学]