荧光免疫分析法测定玉米中赤霉烯酮含量  

Quantitative Determination of Zearalenone Content in Corn Using Fluorescence Immunoassay

作　　者：张艳 王伟 乔澎波 孟祥绍 ZHANG Yan;WANG Wei;QIAO Pengbo;MENG Xiangshao(China Grain Reserves Henan Quality Inspection Center Co.,Ltd.,Zhengzhou 450000,China)

机构地区：[1]中储粮河南质检中心有限公司,河南郑州450000

出　　处：《现代食品》2025年第5期200-202,共3页Modern Food

摘　　要：本文建立一种测定玉米中赤霉烯酮含量的检测方法。通过使用荧光标记的抗体测定荧光强度来确定赤霉烯酮的含量。结果表明,该方法线性关系良好,R2=0.9985;方法检出限为0.5μg·kg^(-1),定量限1.0μg·kg^(-1);加标回收率在85.6%~95.3%,相对标准偏差小于8%。该方法快速、灵敏、特异性强以及准确性良好,适用于测定玉米中的赤霉烯酮含量。A method for the determination of gibberellenone in corn was developed.The amount of gibberellenone was determined by using fluorescent-labeled antibodies and measuring the fluorescence intensity.The results show that the linear relationship of the method is good,R2=0.9985;the limit of detection and limit of quantification were 0.5μg·kg^(-1)and 1.0μg·kg^(-1);the recoveries were 85.6%to 95.3%,and the relative standard deviations were less than 8%.The method was rapid,sensitive,specific and accurate,and was suitable for the determination of gibberellenone in corn.

关 键 词：荧光免疫分析 竞争法 赤霉烯酮 玉米 残留检测 

分 类 号：S481.8[农业科学—农药学]