OSAHS儿童与非OSAHS儿童唾液差异蛋白质组学研究  

Proteomic Study on Salivary Differences between OSAHS Children and Non-OSAHS Children

作　　者：祖丽胡马尔·努尔艾合买提 麦克丽亚·帕力哈提 古力巴哈·买买提力 Zulihumaer·NUERAIHEMAITI;Maikeliya·PALIHATI;Gulibaha·MAIMAITILI(Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063,China)

机构地区：[1]新疆医科大学第二附属医院,新疆乌鲁木齐830063

出　　处：《口腔医学研究》2025年第4期306-313,共8页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81860194);新疆维吾尔自治区自然科学基金(编号:2022D01C275)。

摘　　要：目的:通过蛋白质组学检测技术分析阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome,OSAHS)儿童和非OSAHS儿童唾液差异蛋白的相关信息,为深入研究OSAHS发病机制,探寻OSAHS生物标志物及关键治疗靶点提供研究基础。方法:收集6例OSAHS儿童[男5例、女1例,平均年龄(12.00±2.71)岁]及6例非OSAHS健康儿童[男3例、女3例,平均年龄(10.22±2.05)岁]唾液样本,进行总蛋白质提取,采用数据非依赖采集(data-independent acquisition,DIA)质谱分析和对差异蛋白行蛋白质鉴定,最后对被筛选的差异蛋白进行生物信息学分析。结果:非OSAHS组中有5636个蛋白、OSAHS组中有5608个蛋白被鉴定,其中5582个蛋白在所有组中都存在。筛选出两组之间的显著差异蛋白,303个蛋白上调表达和185个蛋白下调表达,其中筛选了KRT80、CXCL10、KRT19、SAA2等差异显著的蛋白。结论:OSAHS儿童和非OSAHS儿童唾液蛋白表达存在差异,相关分子标志物可能与OSAHS发生、发展、发病机制密切相关。Objective:To analyze the related information of salivary differential proteins between OSAHS children and non-OSAHS children through proteomics detection technology.Methods:Saliva samples were collected from 6 children with OSAHS[5 males and 1 female,with an average age of(12.00±2.71)years]and 6 non-OSAHS healthy children[3 males and 3 females,with an average age of(10.22±2.05)years].Total proteins were extracted,data-independent acquisition(DIA)mass spectrometry analysis and protein identification of differential proteins were performed,and finally bioinformatics analysis was conducted on the screened differential proteins.Results:5636 proteins were identified in the non-OSAHS group and 5608 proteins in the OSAHS group,among which 5582 proteins were present in all groups.A total of 303 up-regulated proteins and 185 down-regulated proteins were screened out as significant differences between the two groups,among which several proteins with significant differences,such as KRT80,CXCL10,KRT19,and SAA2,were selected.Conclusion:There are differences in salivary proteins between OSAHS children and non-OSAHS children,and OSAHS molecular markers may be closely related to its occurrence,development,and pathogenesis.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 唾液蛋白 蛋白质组学 生物信息学分析 DIA技术 差异蛋白 整夜多导睡眠监测 张口呼吸 

分 类 号：R766[医药卫生—耳鼻咽喉科]