共聚焦拉曼光谱在皮肤屏障功能评估和经皮吸收研究中的应用  

Application of confocal Raman spectroscopy in the evaluation of skin barrier function and permeability

作　　者：杨武成 谢宇 魏剑 范瑞芳[3] 谭建华 席绍峰 Wucheng Yang;Yu Xie;Jian Wei;Ruifang Fan;Jianhua Tan;Shaofeng Xi(National Quality Supervision and Testing Center for Cosmetics(Guangzhou),Guangzhou Quality Supervision and Testing Institute,Guangzhou,Guangdong 511447,China;Guangzhou NOI-Quality and Safety,Technology Collaborative Innovation Center,Guangzhou,Guangdong 511447,China;School of Life Sciences,South China Normal University,Guangzhou,Guangdong 510631,China)

机构地区：[1]广州质量监督检测研究院国家化妆品质量检验检测中心(广州),广东广州511447 [2]广州市NQI-质量安全科技协同创新中心,广东广州511447 [3]华南师范大学生命科学学院,广东广州510631

出　　处：《日用化学工业(中英文)》2025年第4期508-515,共8页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金(42377366);广州市科技项目“广州市NQI-质量安全科技协同创新中心”(No.2023B04J0407);广东省药监局科技创新项目(No.2021TDB38)。

摘　　要：在皮肤研究领域,共聚焦拉曼光谱是一项新兴的分析技术。它可以用于皮肤生理学分析,获得有关皮肤分子组成和结构的数据,并且该技术还具有高空间分辨率、非侵入性以及无需提前对目标物质进行标记(如使用荧光染料标记)的优点。基于以上优点,这项技术具有成为实验室标准化分析技术的巨大潜力。但共聚焦拉曼光谱技术目前仍处在一个不断发展和完善的阶段,文章介绍了共聚焦拉曼光谱的原理,重点强调了该技术的优点,并概述了共聚焦拉曼光谱技术在皮肤研究,特别是皮肤屏障功能和活性物质经皮吸收研究中的应用。同时分析了共聚焦拉曼光谱的局限性,并展望了共聚焦拉曼光谱的未来发展趋势。Confocal Raman spectroscopy(CRS)is a new analytical technology in the field of percutaneous absorption,which enables physiology analysis to obtain information on the molecular composition and structure of the skin.Due to the advantages of high spatial resolution,non-invasive and non-pretreatment of sample compared to conventional methods,CRS possesses great potential to become a standardized laboratory analytical technique.Currently,the development and application of CRS is still in a stage of improvement.This review focuses on elucidating the principles and the advantages of this technology,and summarizes the application of CRS technique in skin research,including the skin barrier function assessment and permeation research.Furthermore,the limitations of CRS,such as the attenuation of signal and the interference of skin autofluorescence are analyzed,and the challenges and opportunities in CRS technology are also discussed.

关 键 词：经皮吸收 共聚焦拉曼光谱 皮肤屏障 局限性 

分 类 号：TQ658[化学工程—精细化工]