Deep gene exchange break among Konosirus punctatus populations across the northwestern Pacific inferred from AFLP and ISSR markers  

作　　者：Na Song Yiping Ying Yang Zhang Tianxiang Gao 

机构地区：[1]The Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China [2]Key Laboratory of Sustainable Development of Polar Fishery of Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China [3]Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen,Shenzhen 518101,China [4]Fishery College,Zhejiang Ocean University,Zhoushan 316022,China

出　　处：《Acta Oceanologica Sinica》2025年第1期104-112,共9页海洋学报(英文版)

基　　金：The National Key Research and Development Program of China under contract No.2023YFD2401903.

摘　　要：The correct understanding of fish population structure plays a positive role in their fishery management.The dotted gizzard shad,Konosirus punctatus,is widely distributed in the coastal waters of the northwestern Pacific.With the over-exploitation of economically important fishes,its importance is increasingly prominent.To further examine the population genetic structure of K.punctatus across the northwestern Pacific,the amplified fragment length polymorphism(AFLP)and the inter-simple sequence repeats(ISSRs)were employed to perform genetic variation analysis.The results showed that the combination of polyacrylamide gel electrophoresis and silver staining can effectively detect genetic variation for K.punctatus populations.The average proportions of polymorphic loci were 46.26%and 87.13%for AFLP and ISSR markers,respectively,and the genetic diversity parameters showed no obvious differences among populations.Both analysis molecular variance(AMOVA)and pairwise F_(st)suggested that there was significant genetic differentiation between Chinese and Japanese populations.All samples also clustered into two clades based on the unweighted pair-group method analysis(UPGMA)tree by two markers,which indicated significant genetic differentiation among populations.Consistent with the previous studies,there are two highly differentiated groups at the nuclear gene level and they were suggested to be treated as two separate genetic management units.The results of the present study could provide the genetic management strategy for this important economic species.

关 键 词：Konosirus punctatus population genetic differentiation AFLP ISSR 

分 类 号：S917.4[农业科学—水产科学]