淫羊藿治疗心肌损伤的网络药理学分析  

Network Pharmacology Analysis of Epimedium brevicornu for Treatment of Myocardial Damage

作　　者：刘腾达 马欣原 崔云鹤 李亭昱 杨朔 郭笑[3] 母润红[1] LIU Tengda;MA Xinyuan;CUI Yunhe;LI Tingyu;YANG Shuo;GUO Xiao;MU Runhong(Basic Medical College of Beihua University,Jilin 132013,China;School of Stomatology,Shanxi Medical University,Taiyuan 030001,China;Pharmacy College of Beihua University,Jilin 132013,China)

机构地区：[1]北华大学基础医学院,吉林吉林132013 [2]山西医科大学口腔学院,山西太原030001 [3]北华大学药学院,吉林吉林132013

出　　处：《北华大学学报(自然科学版)》2025年第3期329-334,共6页Journal of Beihua University(Natural Science)

基　　金：吉林省科技发展计划项目(YDZJ202401432ZYTS);北华大学研究生创新计划项目(2023067)。

摘　　要：目的利用网络药理学方法,分析淫羊藿治疗心肌损伤潜在活性成分及作用靶点,探讨淫羊藿改善心肌损伤的潜在机制。方法应用中药系统药理学数据库(TCMSP)筛选淫羊藿活性成分及预测相应靶点,通过OMIM、TTD和GeneCards数据库检索得到心肌损伤靶点,将活性成分靶点和心肌损伤靶点取交集,绘制交叉靶点Venn图;利用STRING平台建立蛋白质相互作用(PPI)网络,利用DAVID数据库微生信平台对淫羊藿治疗心肌损伤靶点进行GO功能分析和KEGG通路富集分析,利用Cytoscape 3.9.1软件绘制“药物-活性成分-靶点-疾病”网络。结果通过口服生物利用度(OB)和类药性(DL)两个参数筛选得到23个活性成分和222个作用靶点,活性成分主要包括槲皮素、木犀草素、谷甾醇、山柰酚等;通过OMIM、TTD和GeneCards数据库筛选得到心肌损伤靶点5608个,Venn分析可知,淫羊藿与心肌损伤共同作用靶点176个,核心靶点主要包括AKT1、IL-6、TNF、TP53、IL-1β等;PPI网络显示,淫羊藿和心肌损伤共同靶点有176个节点和3705条“边”;GO功能分析可知,淫羊藿主要通过对异生物刺激的反应、基因表达的正调控、缺氧反应等生物过程,对核、核膜、细胞外间隙等细胞组分,以及酶结合、蛋白质结合核受体活性等分子功能,发挥治疗心肌损伤作用;KEGG通路富集分析可知,通路主要富集于癌症信号通路、血脂与动脉粥样硬化、PI3K-AKT信号通路等。结论淫羊藿治疗心肌损伤主要是通过多成分、多靶点、多通路发挥治疗作用。Objective To analyze the potential active components and corresponding targets of Epimedium brevicornu for treating myocardial damage using network pharmacology methods,and to explore its potential mechanisms for improving this condition.Methods The TCMSP database was used to screen for the active components of Epimedium brevicornu and predict their corresponding targets.Targets related to myocardial damage were retrieved from OMIM,TTD and GeneCards databases.The intersections of active components targets and myocardial damage targets were used to create crosstarget Venn diagrams.The STRING platform was employed to establish a protein-protein interaction(PPI)network,while the DAVID database was utilized for GO functional analysis and KEGG pathway enrichment analysis of the targets of Epimedium brevicornu in the context of myocardial damage.Finally,the drug-active conponents-targets-disease network was constructed using Cytoscape 3.9.1 software.Results Through the screening of two parameters-oral bioavail ability(OB)and drug-like(DL)properties were obtained 23 active components and 222 corresponding targets.The active components mainly included quercetin,luteolin,sitosterol and kaempferol,among others.By screening the OMIM,TTD and GeneCards databases,we identified 5608 targets related to myocardial damage.Venn analysis revealed that Epimedium brevicornu and myocardial damage jointly acted on 176 common targets,with core targets including AKT1,IL-6,TNF,TP53 and IL-1β.The PPI network indicates that Epimedium brevicornu and myocardial damage have 176 nodes and 3705 edges among their common targets.The GO function analysis showed that Epimedium brevicornu mainly exerts its therapeutic effect on myocardial damage through responses to xenobiotic stimuli,positive regulation of gene expression,hypoxia response and other biological processes.It also affected cellular components such as the nucleus,nuclear membrane and extracellular space,as well as molecular functions like enzyme binding and protein-binding nuclear recept

关 键 词：网络药理学 淫羊藿 心肌损伤 

分 类 号：R285.5[医药卫生—中药学]