Recent technology advancements in large-scale DNA assembly  

作　　者：Jielin Li Li Cheng Yingxin Ma Yizhi Cai Junbiao Dai 

机构地区：[1]Shenzhen Key Laboratory of Synthetic Genomics,Guangdong Provincial Key Laboratory of Synthetic Genomics,Key Laboratory of Quantitative Synthetic Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,China [2]University of Chinese Academy of Sciences,Beijing,China [3]Manchester Institute of Biotechnology,University of Manchester,Manchester,UK [4]Guangdong Laboratory for Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China

出　　处：《Quantitative Biology》2025年第1期143-151,共9页定量生物学(英文版)

基　　金：Elite Young Scientists Program of CAAS;National Natural Science Foundation of China,Grant/Award Numbers:32150025,31725002;Guangdong Provincial Key Laboratory of Synthetic Genomics,Grant/Award Number:2023B1212060054;Shenzhen Outstanding Talents Training Fund;Science and Technology Planning Project of Guangdong Province,Grant/Award Number:2022A0505090009;Bureau of International Cooperation,Chinese Academy of Sciences,Grant/Award Number:172644KYSB20180022;Innovation Program of Chinese Academy of Agricultural Sciences;Shenzhen Science and Technology Program,Grant/Award Number:KQTD20180413181837372;National Key Research and Development Program of China,Grant/Award Number:2022YFF1201800;Shenzhen Key Laboratory of Synthetic Genomics,Grant/Award Number:ZDSYS201802061806209。

摘　　要：The emergence and development of sequencing and DNA synthesis techniques have empowered us with the ability to read and write genomes,thereby enhancing our understanding and manipulation of biological processes.Over the past two decades,numerous genomes ranging from kilobase-sized viruses to megabase-sized bacteria and yeast chromosomes have been artificially synthesized[1].Notably,the Synthetic Yeast Genome Project(Sc2.0)has successfully constructed all the chromosomes of Saccharomyces cerevisiae,with a strain containing 7.5 synthetic chromosomes also being generated[2–11].It is anticipated that collaborative efforts among scientists worldwide will soon lead to the creation of the first eukaryotic cell harboring a fully synthesized genome.Furthermore,synthetic genomes for higher eukaryotes are also emerging,as exemplified by the ongoing initial phase of the Synthetic Moss Genome Project(Syn-Moss)[12].

关 键 词：saccharomyces cerevisiaewith synthetic yeast genome sequencing dna synthesis techniques understanding manipulation biological processesover sequencing techniques constructed all chromosomes read write genomesthereby artificially synthesized notablythe 

分 类 号：Q78[生物学—分子生物学]