滇牡丹成熟胚和叶柄愈伤组织的诱导及培养条件优化  

Callus Induction from Mature Embryo and Petiole of Paeonia delavayi and Optimization of Culture Conditions

作　　者：蒲艳 张鹏远 王娟 张怀璧 郭雪 符家铭 Pu Yan;Zhang Pengyuan;Wang Juan;Zhang Huaibi;Guo Xue;Fu Jiaming(College of Forestry,Southwest Forestry University,Kunming,650224;School of Life Sciences,Southwest Forestry University,Kunming,650224;School of Horticulture,Southwest Forestry University,Kunming,650224;Royal Plant and Food Institute of New Zealand,Palmerston North,11600,New Zealand)

机构地区：[1]西南林业大学林学院,昆明650224 [2]西南林业大学生命科学学院,昆明650224 [3]西南林业大学园林园艺学院,昆明650224 [4]新西兰皇家植物与食品研究所,新西兰北帕默斯顿11600

出　　处：《分子植物育种》2025年第4期1186-1194,共9页Molecular Plant Breeding

基　　金：云南省重大基础专项生物资源数字化开发应用项目(202002AA100007);国家自然科学基金项目(32060089)共同资助。

摘　　要：滇牡丹(Paeonia delavayi)是一种多年生的亚灌木,具有重要的药用、油用及观赏等价值。本研究以滇牡丹成熟胚、叶柄为外植体材料,采用正交试验等方法对影响滇牡丹愈伤组织诱导及继代培养的部分条件、因子(如基本培养基类型,不同激素处理组合,消毒处理条件,采样时间等)进行探索,结果显示,在MS培养基上成熟胚愈伤组织诱导效果最佳,且成熟胚愈伤组织诱导最佳培养基为MS+0.2 mg/L NAA+0.5 mg/L 6-BA+2.0 mg/L 2,4-D,成熟胚愈伤组织在M2培养基中进行继代培养;1年中以4月份采样的叶柄为外植体最易控制污染,叶柄外植体通过75%乙醇浸泡30 s和1.0%NaClO浸泡15 min处理后,污染率仅为3.33%;消毒后的叶柄外植体在进行3 d预培养后,接种至MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.5 mg/L NAA+0.5 g/L PVP培养基上诱导愈伤组织效果最佳。上述结果可为建立滇牡丹组织培养提供支持与帮助。Paeonia delavayi is a perennial subshrub with important medicinal,oil and ornamental values.In this study,mature embryos and petioles of P.delavayi were used as explant materials.The effects of partial conditions and factors on the callus induction and subculture process of P.delavayi(such as basic medium type,different hormone treatment combinations,disinfection treatment conditions and sampling time et.al)were tested by designed orthogonal test.The results showed that the MS medium was the best basic medium for the callus induction,and the optimized formula of the induction medium was MS+0.2 mg/L NAA+0.5 mg/L 6-BA+2.0 mg/L 2,4-D.The mature embryos callus were subcultured in M2 medium.The petiole sampled in April of a year was the best explants to control pollution.Moreover,the pollution rate of petiole explants can be reduced to 3.33%when treated with 75%ethanol for 30 s and then 1.0%NaClO for 15 min.To obtain the best induction result,the process of pre-culture which culturing sterilized petiole explants on MS basis medium for 3 d was necessary,and the treated explants were then transfered to medium with MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.5 mg/L NAA+0.5 g/L PVP.We hope this study could provide supports and help to the tissue culture of P.delavayi.

关 键 词：滇牡丹(Paeonia delavayi) 成熟胚 叶柄 愈伤组织诱导 

分 类 号：S685.11[农业科学—观赏园艺]