基于转录组测序的海南实生油茶不育性现象的解析  

Analysis of Infertility Phenomenon of Hainan Camellia Seed Trees Based on Transcriptome Sequencing

作　　者：沈建才 张诗慧 贾泽冲 高洁 罗升 马晓帆 吴文嫱[1] 胡海燕 刘进平[1] 周扬[1] 黄东益[1] 赖杭桂[1] Shen Jiancai;Zhang Shihui;Jia Zechong;Gao Jie;Luo Sheng;Ma Xiaofan;Wu Wenqiang;Hu Haiyan;Liu Jinping;Zhou Yang;Huang Dongyi;Lai Hanggui(College of Tropical Crops,Hainan University,Haikou,570228)

机构地区：[1]海南大学热带作物学院,海口570228

出　　处：《分子植物育种》2025年第4期1202-1215,共14页Molecular Plant Breeding

基　　金：海南省重点研发计划项目(ZDYF2021XDNY273);中央财政林业科技推广示范资金项目(琼〔2020〕TG03);海南省高新技术产业发展专项(kjcgzh017);国家热带植物种质资源库项目(NTPGRC2022-021)共同资助。

摘　　要：海南油茶实生树群体中普遍存在开花不育现象,严重影响海南油茶树的生产力,其分子机制仍需进一步解析。本研究以海南油茶正常株与不育株花芽发育各阶段的花器官为试验材料,基于转录组测序挖掘影响海南油茶败育的关键调控基因,旨在比较海南油茶正常株和不育株基因差异,分析差异基因的生物学功能,以进一步从分子水平探索海南油茶不育原因。研究结果表明:在总共获取的175200个单基因中有34.46%的单基因功能信息尚不明确,表明关于海南油茶基因功能的研究还不透彻;对比发现6462个基因为海南油茶正常株与不育株花器官发育的差异表达基因(DEGs),通过对挑选的DEGs进行GO注释、KEGG富集等,结果显示海南油茶不育现象以受植物激素信号转导通路和代谢通路调控为主。最后,对所挑选的10个重要DEGs采用RT-qPCR方法进行验证,结果显示,10个基因表达量的差异倍数与转录组所呈现的结果基本相同,表明DEGs是可信的。本研究进一步解析了海南实生油茶不育性的分子机理,对海南油茶研究有一定意义。Flowering sterility is widespread in the Hainan Camellia seed trees,which seriously affects the productivity of Camellia in Hainan,and its molecular mechanism still needs to be further analysis.In this study,the flower organs of normal and sterile plants of Hainan Camellia were used as experimental materials,and the key regulatory genes affecting the abortion of Hainan Camellia were mined based on transcriptome sequencing,aiming to compare the genetic differences between the sterile and fertile strains of Hainan Camellia,and analyze the biological functions of the differential genes,so as to further explore the causes of infertility of Hainan Camellia from the molecular level.The results showed that 34.46%of the 175200 unigenes obtained had unknown functions,indicating that the research on the gene function of Hainan Camellia was not thorough.6462 genes were found to be differential expression genes(DEGs)for flower organ development between normal and sterile plants of Hainan Camellia.GO annotation and KEGG enrichment were carried out on the selected DEGs,and the results showed that the infertility phenomenon of Hainan Camellia was mainly regulated by plant hormone signal transduction pathway and metabolic pathway.Finally,the selected 10 important DEGs were verified by RT-qPCR.The results showed that the multiples of the expression of the 10 genes were basically the same as those presented by the transcriptome,indicating that DEGs were credible.This study further analyzes the molecular mechanism of infertility of Hainan Camellia seed trees,which has certain significance for the study of Hainan Camellia.

关 键 词：海南油茶 不育株 转录组测序 分子机制 

分 类 号：S794.4[农业科学—林木遗传育种]