苦橙新橙皮苷联合间歇运动对心梗大鼠心肌纤维化的影响  

Effects of Bitter Orange Neohesperidin Combined with Intermittent Exercise on Myocardial Fibrosis in Myocardial Infarction Rats

作　　者：王婷婷 张玉良 Wang Tingting;Zhang Yuliang(Zhanjiang University of Science and Technology,Zhanjiang,524000)

机构地区：[1]湛江科技学院,湛江524000

出　　处：《分子植物育种》2025年第4期1285-1294,共10页Molecular Plant Breeding

基　　金：广东省高等教育教学研究改革项目(ZLGC-202302)资助。

摘　　要：本研究旨在探讨苦橙来源的新橙皮苷(neohesperidin,NHP)联合间歇运动对心肌梗死(MI)大鼠模型的治疗效果及其在心肌纤维化中的机制作用。通过实验观察左心室缩短分数(FS)和射血分数(EF),发现新橙皮苷联合间歇运动显著改善了心梗大鼠的心功能。血清心肌损伤标志物(NT-proBNP,CK-MB和cTnI)的水平显著降低,表明心肌损伤得到减轻。同时,心肌组织学分析显示,联合干预减少了心梗引起的纤维化程度,并降低了促炎因子(TNF-α,IL-1β和IL-18)的表达。分子机制研究表明,联合干预显著抑制了TGF-β1/Smad3信号通路,降低了纤维化相关基因(α-SMA,collagenⅠ和collagenⅢ)和基质金属蛋白酶(MMP-2和MMP-9)的表达。本研究结果表明,苦橙来源的新橙皮苷联合间歇运动通过抑制炎症和纤维化基因表达,显著减轻心梗后心肌纤维化,对心梗后的心肌重塑具有潜在治疗价值。This study aims to investigate the therapeutic effects and mechanisms of neohesperidin(NHP)from Citrus aurantium,combined with intermittent exercise,on myocardial fibrosis in a rat model of myocardial infarction(MI).Evaluation of left ventricular fractional shortening(FS)and ejection fraction(EF)revealed that the combination of neohesperidin and intermittent exercise significantly improved cardiac function in MI rats.The serum levels of myocardial injury markers(NT-proBNP,CK-MB,and cTnI)were markedly reduced,indicating alleviation of myocardial damage.Histological analysis demonstrated reduced fibrosis and decreased expression of proinflammatory factors(TNF-α,IL-1β,and IL-18)in myocardial tissue following the combined treatment.Mechanistically,the combined intervention significantly inhibited the TGF-β1/Smad3 signaling pathway and downregulated fibrosis-associated genes(α-SMA,collagenⅠ,and collagenⅢ)as well as matrix metalloproteinases(MMP-2 and MMP-9).These findings suggest that neohesperidin from Citrus aurantium combined with intermittent exercise reduces myocardial fibrosis through inhibition of inflammatory and fibrotic gene expression,highlighting its therapeutic potential for post-MI cardiac remodeling.

关 键 词：苦橙 新橙皮苷 间歇运动 心肌纤维化 TGF-β1/Smad3通路 

分 类 号：R285.5[医药卫生—中药学]