桐花树土壤内生真菌Aspergillus fumigatus 06T03次生代谢产物的分离及其生物活性研究  

Study on the isolation of secondary metabolites and their biological activities of the endophytic fungus Aspergillus fumigatus 06T03 from the soil of Aegiceras corniculatum

作　　者：代子腾 韦霞 刘妍颖 游证喨 吴承华 王娟 沈张扬 幸尚平 黄倩婵 朱丹 DAI Ziteng;WEI Xia;LIU Yanying;YOU Zhengliang;WU Chenghua;WANG Juan;SHEN Zhangyang;XING Shangping;HUANG Qianchan;ZHU Dan(School of Pharmacy,Guangxi Medical University,Nanning 530021,China;Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation,Nanning 530021,China;Guangxi Key Laboratory of Precise Drug Detection and Screening,Nanning 530021,China)

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西生物活性分子研究与评价重点实验室,南宁530021 [3]广西药物精准检测与筛选重点实验室,南宁530021

出　　处：《广西医科大学学报》2025年第2期210-217,共8页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.82160734);广西科技重大专项资助(No.桂科AA22362);中央引导地方科技发展资金资助项目(No.桂科ZY24212031);广西中医药多学科交叉创新团队资助项目(No.GZKJ2305);广西医科大学青年基金项目(No.GXMUYSF202315);大学生创新创业训练项目资助(No.202410598031)。

摘　　要：目的:研究红树植物桐花树土壤内生真菌Aspergillus fumigatus 06T03的次生代谢产物的分离纯化及其潜在的生物活性。方法:菌株采用大米培养基经规模发酵,乙酸乙酯浸泡提取后经减压浓缩得到乙酸乙酯粗提物。采用多种现代色谱技术如硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等对粗提物进行化学成分分离。通过高分辨质谱、核磁共振波谱等综合谱学技术确定单体化合物结构。采用CCK-8法和Griess法初步评估单体化合物的抗肿瘤活性和抗炎活性。结果:从Aspergillus fumigatus 06T03大米发酵产物中分离鉴定出9个化合物,分别为:Monomethylsulochrin(1)、Questin(2)、Demethylsulochrin(3)、Fumiquinazoline C(4)、Fumiquinazoline J(5)、Azaspirofurans A(6)、Pseurotin A(7)、N-[2-(4-hydroxyphenyl)ethenyl]formamide(8)、Pyripyropene A(9)。其中,化合物1~3属于蒽醌类化合物,4~9属于生物碱类化合物。化合物1~9对肝癌细胞(Huh7)、卵巢癌细胞(A2780)、乳腺癌细胞(MDA-MB-231)无明显抗肿瘤活性,化合物1、3、6、7、8和9具有一定抗炎活性。结论:从桐花树土壤内生真菌Aspergillus fumigatus 06T03中分离得到9个单体化合物,除化合物2,其余化合物均为首次从桐花树内生真菌中分离得到,化合物1、3、6、7、8和9具有一定的抗炎活性。本研究丰富了桐花树土壤内生真菌次生代谢产物的化学多样性,为后续深入开发提供了基础数据。Objective:To investigate the isolation and purification of the secondary metabolites and their potential biological activity in the endophytic fungus Aspergillus fumigatus 06T03 from the soil of Aegiceras corniculatum.Methods:The strain was fermented on a large scale using rice medium,and then extracted by soaking ethyl acetate and concentrated under reduced pressure to obtain a crude extract of ethyl acetate.A variety of modern chromatographic techniques such as silica gel column chromatography,Sephadex LH-20 gel column chromatography,high-performance liquid chromatography were used to separate the chemical components of the crude extract.Their structures were determined by using comprehensive spectral techniques such as high-resolution mass spectra(MS)and nuclear magnetic resonance(NMR)spectroscopy.The antineoplastic and anti-inflammatory activities of the monomers were evaluated using cell counting kit-8(CCK-8)assay and Griess assays.Results:Nine compounds were isolated from the rice fermentation products of Aspergillus fumigatus 06T03 and identified as Monomethylsulochrin(1),Questin(2),Demethylsulochrin(3),Fumiquinazoline C(4),Fumiquinazoline J(5),Azaspirofurans A(6),Pseurotin A(7),N-[2-(4-hydroxyphenyl)ethenyl]formamide(8),and Pyripyropene A(9).Among them,compounds 1-3 belonged to anthraquinones,and 4-9 belonged to alkaloids.The results showed that compounds 1-9 failed to exhibit obvious antineoplastic activity against Huh7 cells,ovarian carcinoma cells(A2780),and breast cancer cells(MDA-MB-231),while compounds 1,3,6,7,8,and 9 had certain antiinflammatory activities.Conclusion:Nine monomeric compounds,except compound 2 which has been isolated before,have been isolated for the first time from the endophytic fungus Aspergillus fumigatus 06T03 from the soil of Aegiceras corniculatum.Compounds 1,3,6,7,8,and 9 have certain anti-inflammatory activities.This study enriches the chemical diversity of the secondary metabolites of endophytic fungus from the soil of Aegiceras corniculatum,and provides basic data for sub

关 键 词：桐花树 内生真菌 烟曲霉 次生代谢产物 生物活性 

分 类 号：Q936[生物学—微生物学]