脾脏和肝脏在血管性血友病因子清除中的作用研究  

The roles of spleen and liver in VWF clearance

作　　者：万严 邓巍[1] Wan Yan;Deng Wei(Cyrus Tang Medical Institute and Collaborative Innovation Center of Hematology,Soochow University,Suzhou 214000,China)

机构地区：[1]苏州大学唐仲英医学研究院血液学研究中心,苏州214000

出　　处：《血栓与止血学》2025年第1期1-7,共7页Chinese Journal of Thrombosis and Hemostasis

基　　金：国家自然科学基金(82270125)。

摘　　要：目的探讨清除血管性血友病因子(von Willebrand factor,VWF)的主要器官。方法本研究主要采用荧光标记的VWF嵌合体(fluorescently labeled VWF chimera,FP⁃VWF KI)小鼠模型,通过组胺刺激模拟内源性VWF的释放。免疫荧光技术用于检测VWF在肝脏和脾脏的分布。此外,通过向野生型小鼠注射Cy5标记的人血浆VWF(Cy5⁃labeled human plasma VWF,Cy5⁃pd⁃VWF)研究了外源性VWF在体内的分布情况。结果研究结果表明,组胺刺激后,释放的内源性VWF在脾脏中分布显著增强。与此同时,注射的外源性Cy5⁃pd⁃VWF在肝脏中的积聚更为明显。结论脾脏在小鼠内源性VWF的清除过程中发挥主导作用。Objective To explore the primary organ responsible for the clearance of von Willebrand factor(VWF).Methods The current study primarily utilizes a fluorescently labeled VWF chimera(FP⁃VWF KI)mouse model,simulating the release of endogenous VWF through histamine stimulation.Immunofluorescence techniques were employed to detect the distribution of VWF in the liver and spleen.Furthermore,the study investigates the distribution of exogenous VWF in the body by injecting Cy5⁃labeled human plasma VWF(Cy5⁃pd⁃VWF)into wild⁃type mice.Results Previous studies,primarily based on the input of exogenous VWF,had identified the liver as the main organ for VWF clearance.Our findings indicated that endogenous VWF released by histamine stimulation was more prominently distributed in the spleen,whereas exogenous Cy5⁃pd⁃VWF accumulated mainly in the liver.Conclusion The spleen plays a leading role in the clearance process of endogenous VWF in mice.

关 键 词：VWF清除 脾脏 肝脏 血栓 

分 类 号：Q493.2[生物学—生理学]