中药结合头针运动疗法改善缺血性脑卒中大鼠肢体痉挛  

Chinese herbal prescription combined with head acupuncture exercise therapy improves limb spasticity in rats with ischemic stroke

作　　者：朱传喜 邱龙 李凌絮 汲广成 Zhu Chuanxi;Qiu Long;Li Lingxu;Ji Guangcheng(Changchun University of Chinese Medicine,Changchun 130117,Jilin Province,China;Tongliao Hospital,Tongliao 028006,Inner Mongolia Autonomous Region,China;The Third Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130117,Jilin Province,China)

机构地区：[1]长春中医药大学,吉林省长春市130117 [2]通辽市医院,内蒙古自治区通辽市028006 [3]长春中医药大学附属第三临床医院,吉林省长春市130117

出　　处：《中国组织工程研究》2025年第35期7571-7577,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家重点研发计划项目(2018YFC1706002),项目参与人:汲广成;吉林省科技发展计划项目(20210203061SF,YDZJ202401122ZYTS),项目负责人:汲广成。

摘　　要：背景:中药结合头针运动疗法治疗缺血性脑卒中后肢体痉挛有显著的临床疗效,然而其具体起效机制尚不明确。目的:探讨中药结合头针运动疗法改善脑卒中后肢体痉挛的可能机制。方法:将70只SD大鼠采用随机数字法分为空白组、模型组、运动疗法组、头针组、中药组、中药头针运动组和假手术组,每组10只。除空白组和假手术组外,其余各组大鼠采用大脑中动脉线栓法制作大鼠脑缺血再灌注损伤模型。各组于造模术后第3天开始进行干预。中药组给予大鼠益脑复健方中药灌胃1.5 g/kg;头针组针刺百会及百会左右两侧各2 mm,留针30 min;运动疗法组在小动物跑步机上跑30 min;中药头针运动组给予益脑复健方中药(1.5 g/kg)灌胃后采用头针针刺(同头针组),在带针状态下予小动物跑步机上运动30 min;模型组和假手术组在同等环境下进行抓取,置于大鼠固定器上30 min;空白组不做任何干预。各组干预频率均为1次/d,连续干预7 d。评估各组大鼠治疗前后神经功能和肌张力,观察脑组织的病理变化,检测大脑皮质中谷氨酸脱羧酶67、琥珀酸半醛脱氢酶和γ-氨基丁酸转氨酶蛋白和mRNA的表达。结果与结论:①与模型组相比,中药头针运动组大鼠神经功能缺损评分和Ashworth痉挛程度量表(modified ashworth spasticity scale,MAS)评分降低(P<0.01),琥珀酸半醛脱氢酶、γ-氨基丁酸转氨酶的蛋白和mRNA表达均下降(P<0.01),谷氨酸脱羧酶67的蛋白和mRNA表达均上升(P<0.01);神经元细胞结构较完整,仅可见部分不规则空洞,核固缩有所减轻。②与中药头针运动组相比,运动疗法组大鼠神经功能缺损评分和MAS肌张力评分高(P<0.05),头针组和中药组大鼠MAS肌张力评分高(P<0.05,P<0.01),其他干预组γ-氨基丁酸转氨酶蛋白和琥珀酸半醛脱氢酶、γ-氨基丁酸转氨酶mRNA的表达均上升(P<0.05,P<0.01);中药头针运动组相较于其他干预组神�BACKGROUND:Chinese herbal prescription with head acupuncture exercise therapy has significant clinical efficacy in the treatment of limb spasticity after ischemic stroke;however,the exact mechanism is not clear.OBJECTIVE:To explore the possible mechanism of the combination of Chinese herbal prescription with head acupuncture exercise therapy in improving limb spasticity after stroke.METHODS:Seventy Sprague-Dawley rats were divided into blank,model,exercise therapy,head-acupuncture,Chinese herbal prescription,Chinese herbal prescription with head acupuncture exercise therapy,and sham-operation groups using the random number method,with 10 rats in each group.Except for the blank group and the sham-operation group,a rat model of cerebral ischemia/reperfusion injury was made by using the middle cerebral artery cable embolism method in the rest of the groups.Interventions in each group started on the 3^(rd) day after modeling.In the Chinese herbal prescription group,rats were given 1.5 g/kg Yi’nao Fujian herbal prescription by gavage;in the head acupuncture group,acupuncture was applied to Baihui and 2 mm on the left and right sides of Baihui,and was left in place for 30 minutes;in the exercise therapy group,rats were exercised on a treadmill for 30 minutes;in the Chinese herbal prescription with head acupuncture exercise therapy group,1.5 g/kg Yi’nao Fujian herbal prescription was given by gavage followed by acupuncture treatment identical to that in the head acupuncture group,and then the animals were exercised for 30 minutes on the treadmill while the acupuncture needles remained in place.Rats in the model and sham-operation groups were subjected to grasping under the same environmental conditions and placed on a rat immobilizer for 30 minutes,and the blank group did not have any intervention.The frequency of intervention in each group was once a day for 7 consecutive days.The neurological deficit score and muscle tone were evaluated in each group before and after treatment.Pathological changes in rat brain tis

关 键 词：中药 头针运动疗法 卒中后痉挛 谷氨酸脱羧酶67 琥珀酸半醛脱氢酶 γ-氨基丁酸转氨酶 益脑复健方 脑卒中 肌张力 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R243